CAS No. 128-53-0
| IUPAC Name | 1-ethylpyrrole-2,5-dione | |
|---|---|---|
| CAS Number | 128-53-0 | |
| Molecular Formula |
C6H7NO2 |
|
| Molecular Weight (MW) | 125.13 | |
| SMILES | CCN1C(=O)C=CC1=O | |
| InChIKey | HDFGOPSGAURCEO-UHFFFAOYSA-N |
N-Ethylmaleimide (NEM) is an organic alkylating agent and irreversible inhibitor of cysteine peptidases, operating via covalent alkylation of active-site thiol groups. Through sulfhydryl modification, NEM inactivates endogenous deubiquitinating enzymes (DUBs) to suppress ubiquitin-dependent signaling pathways. Additionally, NEM specifically inhibits phosphate transport across mitochondrial membranes, disrupting oxidative phosphorylation and downstream metabolic phenotypes.
|
Concentration
Volume
Mass
|
|---|
| Solvent | Solubility & Note |
|---|---|
| DMSO |
25 mg/mL
(199.79 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 25 mg/mL |
| Ethanol | 25 mg/mL |
| DMSO |
25 mg/mL
(199.79 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 25 mg/mL |
| Ethanol | 25 mg/mL |
| DMSO |
25 mg/mL
(199.79 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 25 mg/mL |
| Ethanol | 25 mg/mL |
| DMSO |
25 mg/mL
(199.79 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 25 mg/mL |
| Ethanol | 25 mg/mL |
| DMSO |
25 mg/mL
(199.79 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 25 mg/mL |
| Ethanol | 25 mg/mL |
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Pre-aliquoted for Longevity: Solid-state aliquots offer significantly longer shelf life compared to stock solutions, ensuring maximum stability for every use.
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