Cell-free Assays (IC50/Ki) for Methotrexate

Key TargetIC50 (Cell-free)DescriptionReference
PC-3 10.24 ± 2.90 mM Spectrophotometric assay at 340 nm using cell lysate, activity calculated as Units/mL. PMC8655237
FaDu 07.19 ± 3.65 mM Spectrophotometric assay at 340 nm using cell lysate, activity calculated as Units/mL. PMC8655237
A431 30.96 ± 6.90 mM Spectrophotometric assay at 340 nm using cell lysate, activity calculated as Units/mL. PMC8655237
K-562 21.1 ± 12.3 mM Spectrophotometric assay at 340 nm using cell lysate, activity calculated as Units/mL. PMC8655237
MDA-MB-231 08.19 ± 3.04 mM Spectrophotometric assay at 340 nm using cell lysate, activity calculated as Units/mL. PMC8655237

Cell-based Assay (IC50) for Methotrexate

Cell TypeIC50 (Cell-based)DescriptionReference
HUH-7 cells 5.4 μg/mL MTT method PMC9620408
GL261 cells 2.070 µg/mL (4.555 µM) CCK-8 assays PMC12817699
JAR cells 4.40 ± 0.34 μM MTS assay PMC6599650
JEG3 cells 3.80 μM MTS assay PMC6599650
HCT-116 cells 3.09 ± 0.73 ng/mL MTT (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) assay, calculated using non-linear regression analysis in SigmaPlot® ([Vendor]). PMC10710251
Check the Methotrexate product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Jump to