CAS No. 59-05-2
Batch:
Purity:99.79
Chemical Structure
Check the
Methotrexate
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | (2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic acid | |
|---|---|---|
| CAS Number | 59-05-2 | |
| Molecular Formula |
C20H22N8O5 |
|
| Molecular Weight (MW) | 454.44 | |
| SMILES | CN(CC1=CN=C2C(=N1)C(=NC(=N2)N)N)C3=CC=C(C=C3)C(=O)NC(CCC(=O)O)C(=O)O | |
| InChIKey | FBOZXECLQNJBKD-ZDUSSCGKSA-N |
Biological Activity
Methotrexate is an antimetabolite and antifolate compound that competitively inhibits dihydrofolate reductase (DHFR). By forming an inactive ternary complex with DHFR and NADPH, it blocks the synthesis of purine nucleotides and thymidylate, thereby impairing DNA and RNA syntheses. Consequently, methotrexate disrupts essential metabolic pathways in proliferating cells, leading to inhibition of cell growth and induction of apoptosis. [1][2]
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
91 mg/mL
(200.24 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
| DMSO |
91 mg/mL
(200.24 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Pre-aliquoted for Longevity: Solid-state aliquots offer longer shelf life compared to stock solutions.
Looking for a specific aliquot size? Contact your local sales representative or orders@selleckchem.com.
Reference
Jump to
- Methotrexate Mechanism of Action: Dihydrofolate Reductase Inhibition in Lymphocyte Research
- Methotrexate Clinical Trials
- Methotrexate Working Concentration | Cell Culture | Treatment
- Methotrexate Solubility in DMSO
- Methotrexate Storage
- Methotrexate Stability
- Methotrexate Combination Database
- Methotrexate Molecular Weight
- Methotrexate SMILES
- Methotrexate Chemical Structure (2D and 3D)
- Methotrexate IC50 for Cell-based and Cell-free Assays
- Methotrexate Cayman (13960)? Try a better option.
- Methotrexate MedChemExpress (HY-14519)? Try a better option.
- Methotrexate Sigma (PHR1396)? Try a better option.
- Methotrexate APExBIO (A4347)? Try a better option.
- Methotrexate SDS｜MSDS
Note: Technical data last updated: Sep 1, 2026.