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BM213

Cat.No.P1215

BM213, a selective C5aR1 agonist with an EC50 of 59 nM, induces C5aR1-mediated calcium mobilization and pERK1/2 signaling but not β-arrestin recruitment. BM213 is much more stable in serum and displays no cytotoxicity on SHSY-5Y cells.

Amino-acid Sequence

BM213 Amino-acid Sequence

CAS No.

Selleck's BM213 Has Been Cited by 1 Publication

Quality Control

Batch: P121501 Water] 100 mg/mL] false] ] ] false] ] ] false Purity: 98%
98

Chemical Information & Solubility

Storage (From the date of receipt) 3 years-20°C powder

In vitro
Batch:

Water : 100 mg/mL


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In vivo
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Biological Activity

Description BM213, a selective C5aR1 agonist with an EC50 of 59 nM, induces C5aR1-mediated calcium mobilization and pERK1/2 signaling but not β-arrestin recruitment. BM213 is much more stable in serum and displays no cytotoxicity on SHSY-5Y cells.

In vitro
In vitro

BM213 is a potent and stable peptide C5aR1 agonists that display no C5aR2 activity and over 1000-fold selectivity for C5aR1 over C3aR. BM213 induces C5aR1-mediated calcium mobilization and pERK1/2 signaling but not β-arrestin recruitment. BM213 is functionally similar to C5a in human macrophage cytokine release assays and in a murine in vivo neutrophil mobilization assay.
Cell Research Cell lines HMDMs, SHSY-5Y cells
Concentrations 0.1-100 μM
Incubation Time 0-6 h, 24 h
Method

HMDMs are stimulated with LPS (10 ng/mL) alone or in the presence of BM213. The supernatant content of cytokines after 24 h stimulation is quantified using ELISA. For MTT cell survival assay, differentiated SH-SY5Y cells are treated with varying concentrations of BM213 following a 2.5 h incubation.

In Vivo
In vivo

BM213 shows antitumor activity in a mouse model of mammary carcinoma.
Animal Research Animal Models Mammary carcinoma model mice bearing with EMT-6 cells
Dosages 1mg/kg
Administration IOCV

References
  • https://pubmed.ncbi.nlm.nih.gov/34762432/

Product Information

Appearance White Powder
Peptide Content (by %N) ≥80%
Single Impurity (by HPLC) ≤1.0%
Assay (By Anhydrous, Acetic Acid-free) 95.0~105.0%
MS Consistent
Packing plastic vial(dedicated for peptide packing) or glass vial, quantity according to customer's detail requirement.
Storage
(From the date of receipt)		 3 years-20°C powder
Shipping Room temperature shipping(Stability testing shows this product can be shipped without any cooling measures.)