BM213

Catalog No.P1215 Batch:P121501

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Technical Data

Formula
Molecular Weight CAS No.
Solubility (25°C)* In vitro Water 100 mg/mL
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description BM213, a selective C5aR1 agonist with an EC50 of 59 nM, induces C5aR1-mediated calcium mobilization and pERK1/2 signaling but not β-arrestin recruitment. BM213 is much more stable in serum and displays no cytotoxicity on SHSY-5Y cells.
In vitro

BM213 is a potent and stable peptide C5aR1 agonists that display no C5aR2 activity and over 1000-fold selectivity for C5aR1 over C3aR. BM213 induces C5aR1-mediated calcium mobilization and pERK1/2 signaling but not β-arrestin recruitment. BM213 is functionally similar to C5a in human macrophage cytokine release assays and in a murine in vivo neutrophil mobilization assay.[1]
In vivo

BM213 shows antitumor activity in a mouse model of mammary carcinoma.[1]
Density 95.0~105.0%

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    HMDMs, SHSY-5Y cells

  • Concentrations

    0.1-100 μM

  • Incubation Time

    0-6 h, 24 h

  • Method

    HMDMs are stimulated with LPS (10 ng/mL) alone or in the presence of BM213. The supernatant content of cytokines after 24 h stimulation is quantified using ELISA. For MTT cell survival assay, differentiated SH-SY5Y cells are treated with varying concentrations of BM213 following a 2.5 h incubation.
Animal Study:

[1]
  • Animal Models

    Mammary carcinoma model mice bearing with EMT-6 cells

  • Dosages

    1mg/kg

  • Administration

    IOCV

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.