Alamandine is a component of the renin-angiotensin system, which regulates blood pressure.

Amino-acid Sequence

CAS No. 1176306-10-7

Batch: Purity: 99.85%

99.85

Storage (From the date of receipt)	3 years-20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
			1 month-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL ( (116.95 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 100 mg/mL

Ethanol : Insoluble

In vivo
Batch:

Add solvents to the product individually and in order.

Description	Alamandine is a component of the renin-angiotensin system, which regulates blood pressure.

In vitro
In vitro	Alamandine decreases leptin secretion and expression in isolated adipocytes mediated by Gq, c-Src, p38 mitogen-activated protein and IκB activation, also induces nitric oxide expression via inducible nitric oxidase synthase and plasminogen activator inhibitor 1 expression in isolated adipocytes.^[2]
Cell Research	Cell lines	adipocytes isolated from rats, 3T3L-1 adipocytes
	Concentrations	0.01, 0.1, 1, 10, 100, 1000 nM
	Incubation Time	24 h
	Method	Isolated adipocytes are incubated with alamandine for 24 h. Secreted leptin levels are determined using a leptin immunoassay kit according to the manufacturer’s protocol.

In Vivo
In vivo	Moderately concentrated alamandine reduces blood leptin levels in vivo.^[2]
Animal Research	Animal Models	Wistar rats (10-week-old)
	Dosages	576, 57.6, 5.76, or 0.576 µg/kg
	Administration	i.p.

References
[1] Soltani Hekmat A,et al. Circ J. 2017 Feb 24;81(3):405-412. [2] Uchiyama T, et al. PLoS One. 2017 Jun 7;12(6):e0178769.

References

Molecular Formula:	C₄₀H₆₂N₁₂O₉
Molecular Weight:	855
CAS No.:	1176306-10-7
Appearance:	White Powder
Peptide Content (by %N):	≥80%
Single Impurity (by HPLC):	≤1.0%
Assay (By Anhydrous, Acetic Acid-free):	95.0~105.0%
MS:	Consistent
Packing:	plastic vial(dedicated for peptide packing) or glass vial, quantity according to customer's detail requirement.
Storage (From the date of receipt):	3 years-20°C powder
Shipping:	Room temperature shipping(Stability testing shows this product can be shipped without any cooling measures.)