CAS No. 30516-87-1

Batch: Purity:99.90
Check CoA
Zidovudine Chemical Structure
Chemical Structure
Check the Zidovudine product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1-[(2R,4S,5S)-4-azido-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione
CAS Number 30516-87-1
Molecular Formula

C10H13N5O4
Molecular Weight (MW) 267.24
SMILES CC1=CN(C(=O)NC1=O)C2CC(C(O2)CO)N=[N+]=[N-]
InChIKey HBOMLICNUCNMMY-XLPZGREQSA-N

Ordering Information

Biological Activity

Zidovudine is a nucleoside analogue reverse transcriptase inhibitor (NRTI) that targets HIV reverse transcriptase. As a thymidine analogue, it is intracellularly converted to its active triphosphate form, which competes with endogenous nucleotides for incorporation into viral DNA. This leads to DNA chain termination and blocks the reverse transcription of viral RNA into double-stranded DNA, thereby suppressing viral replication. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
🧮 Molarity Calculator

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 53 mg/mL (198.32 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 53 mg/mL
Water 26 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Jump to

Note: Technical data last updated: Sep 1, 2026.