CAS No. 3026500-20-6

Batch: Purity:99.01
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VVD-214 (VVD-133214, RO7589831) Chemical Structure
Chemical Structure
Check the VVD-214 (VVD-133214, RO7589831) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name N-[(E,1S)-1-cyclopropyl-3-methylsulfonylprop-2-enyl]-2-(1,1-difluoroethyl)-4-phenoxypyrimidine-5-carboxamide
CAS Number 3026500-20-6
Molecular Formula

C20H21F2N3O4S
Molecular Weight (MW) 437.46
InChIKey UWPFUBNCDABUEE-SIFUEBAJSA-N

Biological Activity

VVD-is a synthetic, covalent allosteric inhibitor of Werner syndrome (WRN) helicase that irreversibly engages cysteine 727 within the helicase domain. By stabilizing an inactive protein conformation and suppressing ATP hydrolysis and DNA unwinding, VVD-selectively disrupts genome integrity in microsatellite instability-high (MSI-H) cancer cells. Consequently, this target engagement triggers widespread double-stranded DNA breaks, nuclear enlargement, and selective apoptosis in MSI-H tumor cell lines and xenograft models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 87 mg/mL (198.87 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 10 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Baltgalvis KA, et al. Nature. 2024;629(8011):435-442.

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Note: Technical data last updated: Sep 1, 2026.