Cell-free Assays (IC50/Ki) for Tamoxifen

Key TargetIC50 (Cell-free)DescriptionReference
ER αLBD 10.46 nM Competitive binding affinity assay with [3H]-E2 as a tracer Download PDF
PKC β1 kinase 4.9 µM In vitro kinase assay Download PDF
human recombinant CYP1A1 12 µM CYP1A1 activity assay using CEC as substrate, fitted with a two-parameter log-logistic model Download PDF
PI3K α 19 µM ADP-Glo-based biochemical assay Download PDF
PI3K β 14 µM ADP-Glo-based biochemical assay Download PDF

Cell-based Assay (IC50) for Tamoxifen

Cell TypeIC50 (Cell-based)DescriptionReference
MCF7-LEM4 >4 μmol L−1 Cellular viability assay (SRB assay) Download PDF
BT474-shLEM4 120 nmol L−1 Cellular viability assay (SRB assay) Download PDF
MCF-7 cells 13 nM CyQuant fluorescent DNA-binding dye kit Download PDF
MCF-7 cells 6.3 nM ERE-tk109-Luc reporter gene assay, detected by Bright Glo luciferase reagent Download PDF
MCF-7 25.56 µmol/L MTT assay Download PDF
Check the Tamoxifen product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

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