Sorafenib (BAY 43-9006) IC50 for Cell-based and Cell-free Assays

Cell-free Assays (IC₅₀/K_i) for Sorafenib (BAY 43-9006)

Key Target	IC₅₀ (Cell-free)	Description	Reference
VEGFR-2	0.84 mM	Anti-phosphotyrosine antibody with the Alpha Screen system	PMC12973379
VEGFR-2	0.058 µM	VEGFR-2 Kinase Assay Kit, Kinase-Glo MAX reagent, luminescence measurement	Download PDF
VEGFR-2 kinase	56.2 nM	VEGFR-2 Kinase Assay Kit	PMC12377214
VEGFR2 kinase domain	8.99 nM	Human VEGFR-2 (KDR) kinase assay kit, luminescence detection using Kinase-Glo ™MAX reagent	Download PDF
VEGFR-2	0.17 nM	enzyme assay	Download PDF
VEGFR-2	0.088 mM	VEGFR-2 ELISA kit	PMC11539005
VEGFR-2	0.069 µM	Kinase-Glo® MAX reagent, luminescence measurement	PMC11568772
VEGFR-2	0.84 mM	Alpha Screen system using an anti-phosphotyrosine antibody	PMC10695193
VEGFR2 kinase	0.006 nM	Kinase inhibition assay	PMC12718917
PDGFRβ kinase	0.006 nM	Kinase inhibition assay	PMC12718917
c-KIT kinase	0.006 nM	Kinase inhibition assay	PMC12718917
FLT3 kinase	0.022 nM	Kinase inhibition assay	PMC12718917
RAF kinase	6 nM	Kinase inhibition assay	PMC12718917
RET kinase	10 nM	Kinase inhibition assay	PMC12718917
ABL kinase	>600 nM	Kinase inhibition assay	PMC12718917
AXL kinase	>600 nM	Kinase inhibition assay	PMC12718917
CSF-1R kinase	>600 nM	Kinase inhibition assay	PMC12718917
c-MET kinase	>600 nM	Kinase inhibition assay	PMC12718917
EGFR kinase	>600 nM	Kinase inhibition assay	PMC12718917
Src kinase	>600 nM	Kinase inhibition assay	PMC12718917
TIE2 kinase	>600 nM	Kinase inhibition assay	PMC12718917
TKRB kinase	>600 nM	Kinase inhibition assay	PMC12718917
human EGFR-TK	3.5 µM	human EGFR-TK Elisa kit assay	Download PDF
JNK-2	1 µM	simple step ELISA kit for the quantitative measurement of JNK-2 (pT138/Y185) protein	Download PDF
VEGFR2 kinase	0.0643 μM	AlphaScreen system with antiphosphotyrosine antibody	PMC9948161

Cell-based Assay (IC₅₀) for Sorafenib (BAY 43-9006)

Cell Type	IC₅₀ (Cell-based)	Description	Reference
Huh7 cells	4.88 µM	luminescent cell viability assay, nonlinear regression analysis using GraphPad Prism software	Download PDF
A549	4.04 mM	MTT method	PMC12973379
HepG2	4.00 mM	MTT method	PMC12973379
MCF-7	5.58 mM	MTT method	PMC12973379
HCT116	5.05 mM	MTT method	PMC12973379
HuH7	2.33 μM	Cell Viability assay	PMC13129747
HuH7-SR	9.624 μM	Cell Viability assay	PMC13129747
PLC/PRF/5	4.656 μM	Cell Viability assay	PMC13129747
PLC/PRF/5-SR	14.050 μM	Cell Viability assay	PMC13129747
Sorafenib sensitive organoid	3.128 μM	Cell Viability assay	PMC13129747
Acquired sorafenib resistant organoid	30.24 μM	Cell Viability assay	PMC13129747
HepG2	8.09 μM	CCK-8 assay, nonlinear regression model in GraphPad Prism 9.5	PMC13089260
Huh7	6.37 μM	CCK-8 assay, nonlinear regression model in GraphPad Prism 9.5	PMC13089260
HepG2-R	32.71 μM	CCK-8 assay, nonlinear regression model in GraphPad Prism 9.5	PMC13089260
Huh7-R	23.48 μM	CCK-8 assay, nonlinear regression model in GraphPad Prism 9.5	PMC13089260
Huh7 cells	8 μM	CCK-8 assay	PMC12870335
Huh7	25.70 µM	MTT assay	PMC12927806
PLC/PRF/5	18.19 µM	MTT assay	PMC12927806
HepG2 spheroids	12.9 µM	CellTiter-Glo®3D kit viability assay	Download PDF
HepG2/LX-2 spheroids	28.0 µM	CellTiter-Glo®3D kit viability assay	Download PDF
786-O	11.04 µmol/mL	CCK-8 assay	PMC12910798
ACHN	18.32 µmol/mL	CCK-8 assay	PMC12910798
MCF7-PR cells	1.154 µM	MTT assays	Download PDF
MCF-7	4.95 µM	MTT test	Download PDF
A549	6.32 µM	MTT test	Download PDF
PC-3	6.57 µM	MTT test	Download PDF
MCF-10A	21.63 µM	MTT test	Download PDF
MDA-MB-231	9.4 µM	MTT assay, calculated using CompuSyn software	Download PDF
A549	12 µM	MTT assay, calculated using CompuSyn software	Download PDF
Normal gingival fibroblasts	23.1 µM	MTT assay, calculated using CompuSyn software	Download PDF
Huh7	4 µM	2.0 Luminsecent cell viability assay, viability photometrically assessed, IC50 determined graphically.	Download PDF
HepG2	6 µM	2.0 Luminsecent cell viability assay, viability photometrically assessed, IC50 determined graphically.	Download PDF
Snu387	10 µM	2.0 Luminsecent cell viability assay, viability photometrically assessed, IC50 determined graphically.	Download PDF
Snu475	10 µM	2.0 Luminsecent cell viability assay, viability photometrically assessed, IC50 determined graphically.	Download PDF
Huh7	9.143 µmol/L	CCK8 assay	Download PDF
Huh7 (CHAF1B-knockdown)	4.660 µmol/L	CCK8 assay	Download PDF
HepG2	5.201 µmol/L	CCK8 assay	Download PDF
HepG2 (CHAF1B-overexpression)	8.253 µmol/L	CCK8 assay	Download PDF
A498	63.1 µM	sulforhodamine B (SRB) assay	PMC12377214
HepG2	44.7 µM	sulforhodamine B (SRB) assay	PMC12377214
MDA-MB-231	81.3 µM	sulforhodamine B (SRB) assay	PMC12377214
Hep55.1C	12 μM	PrestoBlue HS Cell Viability Reagent	Download PDF
Hep56.1D	10 μM	PrestoBlue HS Cell Viability Reagent	Download PDF
BMKs	14 μM	PrestoBlue HS Cell Viability Reagent	Download PDF
TT cells	10.2 nM	Real-time cell analysis system (xCELLigence System) with nonlinear regression analysis (Sigmoidal dose–response [variable slope])	Download PDF
HepG2	4 μg/mL	CCK8 assay, Annexin V/7-AAD apoptosis detection kit	Download PDF
MHCC-97H	6 μg/mL	CCK8 assay, Annexin V/7-AAD apoptosis detection kit	Download PDF
Huh7	1.5 μM	CCK-8 assay	PMC12659515
MHCC-97H	3.5 μM	CCK-8 assay	PMC12659515
LPHC-6 cells	13.60 µΜ/mL (24h), 7.64 µΜ/mL (48h), 7.36 µΜ/mL (72h)	MTT or CCK8 assay	PMC12522666
HepG2	4.5 μM	CCK-8 assay	PMC12358154
SNU-387	5.5 μM	CCK-8 assay	PMC12358154
Huh7 cells	5.12 µM	CCK-8 assay, dose-response curve fitting	Download PDF
HepG2	9.13 µM	Alamar Blue (AB) assay	Download PDF
BJ	23.71 µM	Alamar Blue (AB) assay	Download PDF
HepG2	0.956 μM	MTT assay	Download PDF
L929	1.504 μM	MTT assay	Download PDF
Huh7-R cells	21.28 μM	CCK-8 assay	Download PDF
Hep-G2-R cells	22.14 μM	CCK-8 assay	Download PDF
p53−/−;Myc hepatoblast	8.5 μM	MTT assay	Download PDF
H22 cells	6.0 μΜ (at 48h), 1.0 μΜ (at 72h)	CCK8 assay	PMC11963799
Hep3B	5.031 μM	CCK-8 assay	Download PDF
HepG2	5.318 μM	CCK-8 assay	Download PDF
MHCC-97H	9.521 μM	CCK-8 assay	Download PDF
Hep3B (sh-β-catenin)	1.703 μM	Cell viability assay (CCK-8 assay)	Download PDF
HepG2 (sh-β-catenin)	3.163 μM	Cell viability assay (CCK-8 assay)	Download PDF
MHCC-97H (sh-β-catenin)	5.042 μM	Cell viability assay (CCK-8 assay)	Download PDF
Huh7	14.86 µM	Cell viability assay	PMC11660897
Huh7R	32.31 µM	Cell viability assay	PMC11660897
Ea.Hy926	10.15 µM	Alamar Blue (AB) protocol	Download PDF
HaCaT	12.87 µM	Alamar Blue (AB) protocol	Download PDF
A375	5.26 µM	Alamar Blue (AB) protocol	Download PDF
Li7 (shNC), Li7 (shALDH1L2-1), Li7 (shALDH1L2-2)	14.23 μM (shNC), 7.05 μM (shALDH1L2-1), 7.41 μM (shALDH1L2-2	Cell viability assay (CCK8 assay)	PMC11648791
SNU387 (shNC), SNU387 (shALDH1L2-1), SNU387 (shALDH1L2-2)	13.65 μM (shNC), 8.21 μM (shALDH1L2-1), 8.58 μM (shALDH1L2-2	Cell viability assay (CCK8 assay)	PMC11648791
Huh7 (Vector), Huh7 (ALDH1L2 overexpression)	10.31 μM (Vector), 17.59 μM (ALDH1L2 overexpression)	Cell viability assay (CCK8 assay)	PMC11648791
MHCC-97H (Vector), MHCC-97H (ALDH1L2 overexpression)	10.59 μM (Vector), 16.57 μM (ALDH1L2 overexpression)	Cell viability assay (CCK8 assay)	PMC11648791
HepG2, Hep3B	6 µM	WST-1 cell proliferation/viability assay	PMC11600669
MCF-7	0.14 mM	MTT assay	PMC11539005
HepG2	0.13 mM	MTT assay	PMC11539005
HCT-116	5.47 µM	MTT test, colorimetric assay	PMC11568772
HePG2	9.18 µM	MTT test, colorimetric assay	PMC11568772
Hela	7.26 µM	MTT test, colorimetric assay	PMC11568772
MCF-7	4.17 µM	MTT test, colorimetric assay	PMC11568772
WI-38	10.65 µM	MTT test, colorimetric assay	PMC11568772
HepG2	6.616 µM	Cell Counting Kit-8 assay (Cytotoxicity assay)	PMC11712087
HepG2S1	14.69 µM	Cell Counting Kit-8 assay (Cytotoxicity assay)	PMC11712087
Huh7	8.61 µM	Cell Counting Kit-8 assay (Cytotoxicity assay)	PMC11712087
Huh7S1	42.58 µM	Cell Counting Kit-8 assay (Cytotoxicity assay)	PMC11712087
HCCLM3	17.04 μM	CCK-8 assay	Download PDF
Huh7	7.322 μM	CCK-8 assay	Download PDF
MHCC97-H	1.02 μmol/L	MTT assay	PMC11589030
LMC-3	0.91 μmol/L	MTT assay	PMC11589030
HepG2	1.16 μmol/L	MTT assay	PMC11589030
HepG2 2.2.15	1.25 μmol/L	MTT assay	PMC11589030
MHCC97-H	0.36 μmol/L	MTT assay	PMC11589030
Huh7	4.0 mM	previously described methods (34)	Download PDF
HepG2	3.6 mM	previously described methods (34)	Download PDF
Huh7/SOR	11.3 mM	previously described methods (34)	Download PDF
HepG2/SOR	13.2 mM	previously described methods (34)	Download PDF
SMMC-7721 cells	8.35 μM	CCK-8 method and GRcalculator tools for 24 h treatment	Download PDF
HLF	5 µM	CCK-8 assay (analyzed at 450 nM)	Download PDF
HuH-6	8 µM	CCK-8 assay (analyzed at 450 nM)	Download PDF
Molm13 (Vector)	15.2 nM	CCK-8 assay	PMC11304842
Molm13 (SPHK1 OE)	30.1 nM	CCK-8 assay	PMC11304842
MV4-11 (Vector)	14.8 nM	CCK-8 assay	PMC11304842
MV4-11 (SPHK1 OE)	29.7 nM	CCK-8 assay	PMC11304842
4T1 cells	37.68 μm	MTT assay	Download PDF
HepG2	7.56 μM (sensitive), 16.51 μM (resistant)	IC50 measurement	Download PDF
HepG2	~7.5 μM	IC50 measurement	Download PDF
HepG2 (GCN5L1 KO)	~3.5 μM	IC50 measurement	Download PDF
SNU-449 wild-type cells	1.98 µM	cell counting kit-8 assay (CCK-8)	PMC11351498
SNU-449 sorafenib-resistant cells	6.518 µM	cell counting kit-8 assay (CCK-8)	PMC11351498
MHCC-97H	10 mM	Cell Counting Kit-8 (CCK-8) assay	Download PDF
HCC-LM3	10 mM	Cell Counting Kit-8 (CCK-8) assay	Download PDF
Huh7	10.83 uM	CCK-8 reagent	Download PDF
HepG2	7.59 uM	CCK-8 reagent	Download PDF
4T1 cells	101.4 µM	MTT kit	Download PDF
SW620B8-mCherry	7.1 µM	trypan blue exclusion in Neubauer chamber	Download PDF
SW480B8-mCherry	14.9 µM	trypan blue exclusion in Neubauer chamber	Download PDF
HEK 293T	32.7 µM	trypan blue exclusion in Neubauer chamber	Download PDF
Huh7	5 μM	CCK-8 assay	PMC10777069
Hep3B	4.3 μM	CCK-8 assay	PMC10777069
HepG2	2µM	MTT assay	PMC11090194
HLF	2µM	MTT assay	PMC11090194
HLE	2µM	MTT assay	PMC11090194
Huh 7 PX	10µM	MTT assay	PMC11090194
Huh 7.5	10µM	MTT assay	PMC11090194
SK Hep1	10µM	MTT assay	PMC11090194
Huh 7	2-10µM	MTT assay	PMC11090194
Huh 7AF	2-10µM	MTT assay	PMC11090194
Huh 7.5.1	2-10µM	MTT assay	PMC11090194
MCF-7	4.45 μM	MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay	Download PDF
HCCLM3-control-shRNA cells	10.57 μM	MTT assay	Download PDF
HCCLM3-UBE2S-shRNA cells	8.68 μM	MTT assay	Download PDF
Bel-7402 (Mock)	15.87 μM	MTT assay	Download PDF
Bel-7402 (pUBE2S)	24.57 μM	MTT assay	Download PDF
8305c	12 μM	XTT viability assay	Download PDF
8505c	5.3 μM	XTT viability assay	Download PDF
ACT-1	15 μM	XTT viability assay	Download PDF
B-CPAP	0.4 μM	XTT viability assay	Download PDF
CAL-62	9 μM	XTT viability assay	Download PDF
FTC-238	6.5 μM	XTT viability assay	Download PDF
MDA-T32	4.5 μM	XTT viability assay	Download PDF
SW1736	8.1 μM	XTT viability assay	Download PDF
A549	4.04 mM	MTT assay	PMC10695193
MCF-7	5.58 mM	MTT assay	PMC10695193
HCT-116	5.05 mM	MTT assay	PMC10695193
HepG2	4.00 mM	MTT assay	PMC10695193
Hep3B	4.2 μM	CCK-8 assay	Download PDF
PLC	10.5 μM	CCK-8 assay	Download PDF
Huh7	18.6 μM	CCK-8 assay	Download PDF
Hep3B (PAK1-overexpression)	10.1 μM	CCK-8 assay	Download PDF
PLC (treated with FRAX597)	4.8 μM	CCK-8 assay	Download PDF
Huh7 (treated with FRAX597)	8.5 μM	CCK-8 assay	Download PDF
HepG2	22.81 μM	Anti-proliferative assay	PMC12330989
Huh-7	11.45 μM	Anti-proliferative assay	PMC12330989
Hep3B	12.02 μM	Anti-proliferative assay	PMC12330989
SNU-449	29.45 μM	Anti-proliferative assay	PMC12330989
SNU-398	19.72 μM	Anti-proliferative assay	PMC12330989
SNU-423	35.69 μM	Anti-proliferative assay	PMC12330989
DES9525	11 mmol/L	CyQuant Cell Proliferation Assay Kit (DNA content reduction)	PMC10792363
DES8163	13 mmol/L	CyQuant Cell Proliferation Assay Kit (DNA content reduction)	PMC10792363
H1299 cells	14.05 μM (alone); 2.34 μM (with DFIQ)	MTT assay (for cell viability)	Download PDF
G/7;7	29μT	OI/93 activity determination	Download PDF
/7;J	29μT	OI/93 activity determination	Download PDF
NC_PLC	7.265 mM	CCK-8 assay	Download PDF
resis-PLC	11.01 mM	CCK-8 assay	Download PDF
resis-PLC with YWHAB knockdown	4.288 mM	CCK-8 assay	Download PDF
A549 cells	95 μM	Virus replication assay (plaque assay for virus load, MTS assay for cell viability)	Download PDF
A549 cells	95 μM	Virus replication assay	Download PDF
HepG2	10.83 µM	MTT assay, nonlinear regression analysis	Download PDF
MDA-MB-231	23.69 µM	MTT assay, nonlinear regression analysis	Download PDF
MHCC97L (PFKFB4 overexpression, Normoxia)	16.35 uM	MTT assay	PMC10140800
SW837	6.86 µM	MTT assay	Download PDF
SMMC-7721-WT	2.46 μg/mL	CCK8 assay	Download PDF
SMMC-7721-SR	14.78 μg/mL	CCK8 assay	Download PDF
HepG2 cells	29.5 μg/mL	CCK-8 assays	Download PDF
Huh-7	7.026 μM	CKK-8 assay	Download PDF
Huh-7SR	27.733 μM	CKK-8 assay	Download PDF
Hep-3B cells	12.73 μM	CCK8 assay	PMC10293458
BEL-7405 cells	10.64 μM	CCK8 assay	PMC10293458
HepG2	7.1 μM	MTT assay	Download PDF
HepG2	> 15 μM	MTT assay	Download PDF
Hep3B	13.7 μM	MTT assay	Download PDF
Hep3B	17.1 μM	MTT assay	Download PDF
Huh7	4.2 μM	MTT assay	Download PDF
Huh7	12 μM	MTT assay	Download PDF
HepG2	10 µM	MTT assay	Download PDF
Huh7	10 µM	MTT assay	Download PDF
SR-HepG2	35 µM	MTT assay	Download PDF
SR-Huh7	35 µM	MTT assay	Download PDF
PLC/PRF/5	6–8 µM	MTT assay	PMC9635392

Check the Sorafenib (BAY 43-9006) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Size	Price	Qty
20mg	USD 147
50mg	USD 270
200mg	USD 497

Cell-free Assays (IC50/Ki) for Sorafenib (BAY 43-9006)

Cell-based Assay (IC50) for Sorafenib (BAY 43-9006)

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Cell-free Assays (IC₅₀/K_i) for Sorafenib (BAY 43-9006)

Cell-based Assay (IC₅₀) for Sorafenib (BAY 43-9006)