CAS No. 139755-83-2

Batch: Purity:99.96
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Sildenafil Chemical Structure
Chemical Structure
Check the Sildenafil product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 139755-83-2
Molecular Formula

C22H30N6O4S
Molecular Weight (MW) 474.58
SMILES CCCC1=NN(C2=C1N=C(NC2=O)C3=C(C=CC(=C3)S(=O)(=O)N4CCN(CC4)C)OCC)C

Biological Activity

Sildenafil is a potent and selective phosphodiesterase 5 (PDE5) inhibitor with an IC50 of. By blocking PDE5-mediated hydrolytic degradation of cyclic guanosine monophosphate (cGMP), it increases intracellular cGMP levels. This accumulation promotes activation of cGMP-dependent protein kinase (PKG) signaling, leading to vascular smooth muscle relaxation and vasodilation across dependent tissues. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 50 mg/mL (105.35 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
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Reference

[1] Ballard SA, et al. J Urol. 1998;159(6):2164-71.

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Note: Technical data last updated: Sep 1, 2026.