Cell-free Assays (IC50/Ki) for Regorafenib (BAY 73-4506)

Key TargetIC50 (Cell-free)DescriptionReference
EGFR kinase 0.7186 μM Glo-kinase assay system Download PDF
RET, RAF1, VEGFR2, c-kit, VEGFR1, PDGFR β, VEGFR3, FGFR1 1.5 nM (RET), 2.5 nM (RAF1), 4.2 nM (VEGFR2), 7 nM (c-kit), cell-free assays Download PDF
Ret, Raf-1, VEGFR2, c-Kit Ret: 1.5 nM, Raf-1: 2.5 nM, VEGFR2: 4.2 nM, c-Kit: 7 nM kinase phosphorylation assay PMC7987272
PDGFR β 22 nM IC50 determination for PDGFR β inhibition Download PDF
VEGFR-1 0.013 µmol/L Kinase inhibition assay Download PDF

Cell-based Assay (IC50) for Regorafenib (BAY 73-4506)

Cell TypeIC50 (Cell-based)DescriptionReference
HT29-P 0.055±0.009 μM MTT assay, nonlinear regression using GraphPad Prism 9 PMC13034650
HT29-R 0.662±0.05 μM MTT assay, nonlinear regression using GraphPad Prism 9 PMC13034650
WiDr-P 0.058±0.009 μM MTT assay, nonlinear regression using GraphPad Prism 9 PMC13034650
WiDr-R 0.723±0.08 μM MTT assay, nonlinear regression using GraphPad Prism 9 PMC13034650
MG63 cells 26 ± 3 µM MTS assay, calculated using AAT Bioquest IC50 Calculator and verified in GraphPad software by applying nonlinear regression using a four-parameter logistic model (log[inhibitor] vs. response, variable slope). PMC13094573
Check the Regorafenib (BAY 73-4506) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Jump to