SMILES:

CC1=NC=C(C(=C1O)C=O)COP(=O)(O)O
Pyridoxal 5′-phosphate hydrate Chemical Structure
Chemical Structure
Check the Pyridoxal 5′-phosphate hydrate product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (4-formyl-5-hydroxy-6-methyl-3-pyridinyl)methyl dihydrogen phosphate
CAS Number 853645-22-4
Molecular Formula

C8H10NO6P.xH2O

Molecular Weight (MW) 247.14
SMILES CC1=NC=C(C(=C1O)C=O)COP(=O)(O)O
InChIKey NGVDGCNFYWLIFO-UHFFFAOYSA-N

How to Prepare Stock Solution?

Concentration Volume Mass
🧮 Molarity Calculator

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 49 mg/mL (198.26 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 6 mg/mL
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Aliquotting Service

Free Aliquotting Service

Pre-aliquoted for Longevity: Solid-state aliquots offer significantly longer shelf life compared to stock solutions, ensuring maximum stability for every use.

Looking for a specific aliquot size? Reach out to your local sales representative or contact us at orders@selleckchem.com.

Note: Technical data last updated: Sep 1, 2026.