CAS No. 66-27-3

Batch: Purity:99
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Methyl methanesulfonate Chemical Structure
Chemical Structure
Check the Methyl methanesulfonate product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name methyl methanesulfonate
CAS Number 66-27-3
Molecular Formula

C2H6O3S
Molecular Weight (MW) 110.13
SMILES CO[S](C)(=O)=O
InChIKey MBABOKRGFJTBAE-UHFFFAOYSA-N

Ordering Information

Biological Activity

Methyl methanesulfonate is a DNA alkylating agent that directly modifies guanine to-methylguanine and adenine to-methyladenine. These primary base modifications cause base mispairing and induce replication blocks, triggering cellular activation of the base excision repair (BER) pathway. Persistent or unrepaired alkylation damage leads to stalled replication forks, accumulation of DNA single-strand breaks, and cytotoxicity. [1][2]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.