CAS No. 87081-35-4
Batch:
Purity:99.74
Chemical Structure
Check the
Leptomycin B (LMB)
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | (2E,5S,6R,7S,9R,10E,12E,15R,16Z,18E)-17-ethyl-6-hydroxy-3,5,7,9,11,15-hexamethyl-19-[(2S,3S)-3-methyl-6-oxo-2,3-dihydropyran-2-yl]-8-oxononadeca-2,10,12,16,18-pentaenoic acid | |
|---|---|---|
| CAS Number | 87081-35-4 | |
| Molecular Formula |
C33H48O6 |
|
| Molecular Weight (MW) | 540.73 | |
| InChIKey | YACHGFWEQXFSBS-XYERBDPFSA-N |
Biological Activity
Leptomycin B is an antifungal antibiotic that acts as a potent, covalent inhibitor of the nuclear export factor CRM1 (XPO1), inhibiting HIV-1 Rev nuclear export with an IC50 of. By alkylating a conserved cysteine residue in CRM1, it blocks NES-mediated nuclear export of target proteins. This disrupts nucleocytoplasmic transport, promoting nuclear retention of tumor suppressors and triggering cell cycle arrest and apoptosis in cancer cells. [1][2]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
100 mg/mL
(184.93 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 100 mg/mL |
| Water | Insoluble |
| DMSO |
100 mg/mL
(184.93 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 100 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
Jump to
- Leptomycin B (LMB) Mechanism of Action: CRM1 Inhibition in Cancer Cell Research
- Leptomycin B (LMB) Clinical Trials
- Leptomycin B (LMB) Working Concentration | Cell Culture | Treatment
- Leptomycin B (LMB) Solubility in DMSO
- Leptomycin B (LMB) Stock Solution
- Leptomycin B (LMB) Storage
- Leptomycin B (LMB) Stability
- Leptomycin B (LMB) Combination Database
- Leptomycin B (LMB) Molecular Weight
- Leptomycin B (LMB) Chemical Structure (2D and 3D)
- Leptomycin B (LMB) IC50 for Cell-based and Cell-free Assays
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Note: Technical data last updated: Sep 1, 2026.