| Form | Storage Temp | Shelf Life |
|---|---|---|
| 3 years-20°C powder | ||
| In Solvent | prepare solutions fresh‑for‑use | |
Cycloheximide is sensitive to light. Store protected from light and minimize exposure to light. Prepare solutions fresh prior to use; stock-solution storage is not recommended.
Check the
Cycloheximide
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
56 mg/mL
(199.04 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 56 mg/mL |
| Water | 15 mg/mL |
| DMSO |
56 mg/mL
(199.04 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 56 mg/mL |
| Water | 15 mg/mL |
| DMSO |
56 mg/mL
(199.04 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 56 mg/mL |
| Water | 15 mg/mL |
| DMSO |
56 mg/mL
(199.04 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 56 mg/mL |
| Water | 15 mg/mL |
| DMSO |
56 mg/mL
(199.04 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 56 mg/mL |
| Water | 15 mg/mL |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Pre-aliquoted for Longevity: Solid-state aliquots offer significantly longer shelf life compared to stock solutions, ensuring maximum stability for every use.
Looking for a specific aliquot size? Reach out to your local sales representative or contact us at orders@selleckchem.com.
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- Cycloheximide Working Concentration | Cell Culture | Treatment
- Cycloheximide Solubility in DMSO
- Cycloheximide Stability
- Cycloheximide Combination Database
- Cycloheximide Molecular Weight
- Cycloheximide SMILES
- Cycloheximide CAS Number
- Cycloheximide Chemical Structure (2D and 3D)
- Cycloheximide IC50 for Cell-based and Cell-free Assays
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Note: Technical data last updated: Sep 1, 2026.