CAS No. 66-81-9
Batch:
Purity:99.96
Chemical Structure
Check the
Cycloheximide
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | 4-[(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl]piperidine-2,6-dione | |
|---|---|---|
| CAS Number | 66-81-9 | |
| Molecular Formula |
C15H23NO4 |
|
| Molecular Weight (MW) | 281.35 | |
| SMILES | CC1CC(C(=O)C(C1)C(CC2CC(=O)NC(=O)C2)O)C | |
| InChIKey | YPHMISFOHDHNIV-FSZOTQKASA-N |
Cycloheximide is an antifungal antibiotic and potent eukaryotic protein synthesis inhibitor that targets the 80S ribosome. By binding to the ribosomal E-site on the 60S subunit, it interferes with deacetylated tRNA binding and blocks the translocation step during translational elongation. Consequently, cycloheximide halts total protein synthesis in eukaryotic cells, leading to downstream suppression of ferroptosis and inhibition of autophagy. [1]
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
56 mg/mL
(199.04 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 56 mg/mL |
| Water | 15 mg/mL |
| DMSO |
56 mg/mL
(199.04 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 56 mg/mL |
| Water | 15 mg/mL |
| DMSO |
56 mg/mL
(199.04 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 56 mg/mL |
| Water | 15 mg/mL |
| DMSO |
56 mg/mL
(199.04 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 56 mg/mL |
| Water | 15 mg/mL |
| DMSO |
56 mg/mL
(199.04 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 56 mg/mL |
| Water | 15 mg/mL |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Pre-aliquoted for Longevity: Solid-state aliquots offer significantly longer shelf life compared to stock solutions, ensuring maximum stability for every use.
Looking for a specific aliquot size? Reach out to your local sales representative or contact us at orders@selleckchem.com.
- Cycloheximide Mechanism of Action: 80S Ribosome Inhibition in Eukaryotic Cell Research
- Cycloheximide Clinical Trials
- Cycloheximide Working Concentration | Cell Culture | Treatment
- Cycloheximide Solubility in DMSO
- Cycloheximide Storage
- Cycloheximide Stability
- Cycloheximide Combination Database
- Cycloheximide Molecular Weight
- Cycloheximide SMILES
- Cycloheximide Chemical Structure (2D and 3D)
- Cycloheximide IC50 for Cell-based and Cell-free Assays
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Note: Technical data last updated: Sep 1, 2026.