Cycloheximide is a potent, broad-spectrum 80S ribosome inhibitor. It affects p53, NF-κB and apoptosis signaling pathways by binding to the ribosome E site and blocking tRNA translocation, effectively inhibiting de novo protein synthesis and altering protein stability.
Cycloheximide IC50 for Cell-based and Cell-free Assays
Comprehensive IC50 Values for Cycloheximide Across Cell-free Enzyme Inhibitory Profiles and Cell-based Functional Assays.