CAS No. 57-00-1

Batch: Purity:98
Creatine Chemical Structure
Chemical Structure
Check the Creatine product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 57-00-1
Molecular Formula

C4H9N3O2

Molecular Weight (MW) 131.13
SMILES CN(CC(=O)O)C(=N)N

Ordering Information

Biological Activity

Creatine is an endogenous nitrogenous organic acid that plays a key role in cellular energy metabolism. It facilitates the rapid recycling of adenosine triphosphate (ATP) primarily in skeletal muscle and brain tissues. Through the creatine kinase system, creatine maintains cellular energy homeostasis, buffers ATP levels during high metabolic demand, and supports downstream physiological functions in neural and muscular tissues. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 18 mg/mL (超声加热十分钟,60℃)
DMSO Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.