CAS No. 64-86-8

Batch: Purity:99.99
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Colchicine Chemical Structure
Chemical Structure
Check the Colchicine product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[a]heptalen-7-yl]acetamide
CAS Number 64-86-8
Molecular Formula

C22H25NO6
Molecular Weight (MW) 399.44
SMILES CC(=O)NC1CCC2=CC(=C(C(=C2C3=CC=C(C(=O)C=C13)OC)OC)OC)OC
InChIKey IAKHMKGGTNLKSZ-INIZCTEOSA-N

Biological Activity

Colchicine is a potent microtubule polymerization inhibitor that disrupts microtubule dynamics to inhibit cell division and mitosis. By disrupting microtubule assembly, colchicine impairs endothelial cell adhesiveness and suppresses the surface expression of L-selectin on neutrophils. These downstream functional effects impair leukocyte adhesion, recruitment, and inflammatory signalling pathways without inducing direct cytotoxicity or reducing cell viability. [1][2]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 80 mg/mL (200.28 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 80 mg/mL
Water 79 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Skoufias DA, et al. Biochemistry. 1992;31(3):738-46.

[2] Cronstein BN, et al. J Clin Invest. 1995;96(2):994-1002.

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Note: Technical data last updated: Sep 1, 2026.