CAS No. 59-14-3

Batch: Purity:99.99
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BrdU (Bromodeoxyuridine, 5-BrdU) Chemical Structure
Chemical Structure
Check the BrdU (Bromodeoxyuridine, 5-BrdU) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 5-bromo-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione
CAS Number 59-14-3
Molecular Formula

C9H11BrN2O5
Molecular Weight (MW) 307.1
SMILES C1C(C(OC1N2C=C(C(=O)NC2=O)Br)CO)O
InChIKey WOVKYSAHUYNSMH-RRKCRQDMSA-N

Biological Activity

BrdU (Bromodeoxyuridine) is a synthetic thymidine analog that functions as a DNA synthesis marker by competing with endogenous thymidine for incorporation into replicating DNA. During the S-phase of the cell cycle, cellular machinery incorporates BrdU into newly synthesized genomic DNA strands. This incorporation enables the quantitative detection and measurement of actively proliferating cells in cellular proliferation and cell cycle assays. [1][2]

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 61 mg/mL (198.63 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 3 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
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Reference

[1] Gratzner HG. Science. 1982;218(4571):474-5.

[2] Dolbeare F, et al. Proc Natl Acad Sci U S A. 1983;80(18):5573-7.

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Note: Technical data last updated: Sep 1, 2026.