CAS No. 1129403-56-0

Batch: Purity:99.16
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Amcasertib (BBI503) Chemical Structure
Chemical Structure
Check the Amcasertib (BBI503) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name N-[2-(diethylamino)ethyl]-2,4-dimethyl-5-[(Z)-[2-oxo-5-(2-phenyl-1,3-thiazol-4-yl)-1H-indol-3-ylidene]methyl]-1H-pyrrole-3-carboxamide
CAS Number 1129403-56-0
Molecular Formula

C31H33N5O2S
Molecular Weight (MW) 539.69
SMILES CCN(CC)CCNC(=O)C1=C(NC(=C1C)C=C2C3=C(C=CC(=C3)C4=CSC(=N4)C5=CC=CC=C5)NC2=O)C
InChIKey QDWKGEFGLQMDAM-ULJHMMPZSA-N

Biological Activity

Amcasertib (BBI503) is a first-in-class cancer stemness kinase inhibitor designed to target stemness pathways in cancer stem cells. By inhibiting stemness kinases, Amcasertib suppresses the expression of Nanog and other critical stemness factors. This inhibition disrupts cancer stem cell self-renewal and stemness maintenance, ultimately impairing tumor-initiating capacity, preventing stemness-driven tumor relapse, and suppressing cell proliferation across various cancer models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 14 mg/mL (25.94 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 1 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Yang L, et al. Signal Transduct Target Ther. 2020;5(1):8.

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Note: Technical data last updated: Sep 1, 2026.