PF-02341066 (Crizotinib) IC50 for Cell-based and Cell-free Assays

Cell-free Assays (IC₅₀/K_i) for PF-02341066 (Crizotinib)

Key Target	IC₅₀ (Cell-free)	Description	Reference
LRRK2 G2019S	980 nM	P81 ﬁlter-binding method	Download PDF
ROS-1	253.7 nM	Inhibitory activity assay	Download PDF
ROS1 kinase	40–60 nM	Kinase activity assay	PMC7283026
ALK kinase	150.8 nM	in vitro ALK inhibition assay	PMC6699152
MST1R	200nM	MST1R inhibition assay, KINOMEscan	PMC5438060
ROS1 kinase	1.7 nM	Kinase activity assay (implied by IC50 measurement for kinase)	Download PDF
MTH1 (with 8-oxo-dGTP)	330 nM	Luminescence-based assay (PPiLight Inorganic Pyrophosphate Assay kit) monitoring pyrophosphate generation, non-linear regression analysis using GraphPad Prism.	PMC4150021
c-Met	4 nM	Inhibitor potency assay	Download PDF

Cell-based Assay (IC₅₀) for PF-02341066 (Crizotinib)

Cell Type	IC₅₀ (Cell-based)	Description	Reference
MET	11 nmol/L	Cell-based assays	Download PDF
SU-DIPG-36 cells	31.54 µM	Multi-well crystal violet (CV) assay	Download PDF
First pATC culture with STRN–ALK fusion	72 nM	WST-1 assay, cell counting, Hoechst 33342 staining, Annexin V staining, Transwell Permeable Supports (migration/invasion)	Download PDF
Second pATC culture with STRN–ALK fusion	85 nM	WST-1 assay, cell counting, Hoechst 33342 staining, Annexin V staining, Transwell Permeable Supports (migration/invasion)	Download PDF
pATC culture without STRN–ALK fusion	112 nM	WST-1 assay, cell counting, Hoechst 33342 staining, Annexin V staining, Transwell Permeable Supports (migration/invasion)	Download PDF
Mlg cells	7.45 μM	MTT assay	PMC12972922
MUG-Chor1	4.57 µM	non-linear regression with a four-parameter logistic model, viability measured by metabolic ATP levels (assay)	Download PDF
U-CH2	6.79 µM	non-linear regression with a four-parameter logistic model, viability measured by metabolic ATP levels (assay)	Download PDF
ADK-VR2	4 nM	interpolation of the growth percentages with a sigmoid dose-response curve by Prism 5 software and IC50 Calculator \| AAT Bioquest	PMC9742620
ADK-VR2 AG143	23.6 nM	interpolation of the growth percentages with a sigmoid dose-response curve by Prism 5 software and IC50 Calculator \| AAT Bioquest	PMC9742620
NL20 cells (EML4-ALK V3a)	234.8 ± 10.8 nM	Dose-dependent ALK activity responses followed with anti-pALK-Y1604 as readout, inhibition curves generated from immunoblotting results, and IC50 values calculated by fitting data to a log (inhibitor concentration) vs. normalized response (variable slope)	Download PDF
NL20 cells (EML4-ALK V3b)	94.8 ± 4.5 nM	Dose-dependent ALK activity responses followed with anti-pALK-Y1604 as readout, inhibition curves generated from immunoblotting results, and IC50 values calculated by fitting data to a log (inhibitor concentration) vs. normalized response (variable slope)	Download PDF
NL20 cells (EML4-ALK (F1174S) V3a)	66.1 ± 0.8 nM	Dose-dependent ALK activity responses followed with anti-pALK-Y1604 as readout, inhibition curves generated from immunoblotting results, and IC50 values calculated by fitting data to a log (inhibitor concentration) vs. normalized response (variable slope)	Download PDF
NL20 cells (EML4-ALK (F1174S) V3b)	71.1 ± 6.1 nM	Dose-dependent ALK activity responses followed with anti-pALK-Y1604 as readout, inhibition curves generated from immunoblotting results, and IC50 values calculated by fitting data to a log (inhibitor concentration) vs. normalized response (variable slope)	Download PDF
MDA-MB-231 parental cells	2.14 μM	Cell viability assay	Download PDF
MDA-MB-231-R	11.3 μM	Cell viability assay	Download PDF
Hs578T parental cells	3.283 μM	Cell viability assay	Download PDF
Hs578T-R	17.71 μM	Cell viability assay	Download PDF
H3122	0.008 μM	assay	PMC9633314
RBE	5.572 µM	WST-1 assay; GraphPad Prism 5 (nonlinear curve ﬁt of Y = 100/(1 + 10((Log10IC50-X)*HillSlope)))	Download PDF
TFK-1	12.79 µM	WST-1 assay; GraphPad Prism 5 (nonlinear curve ﬁt of Y = 100/(1 + 10((Log10IC50-X)*HillSlope)))	Download PDF
HuCCA-1	3.711 µM	WST-1 assay; GraphPad Prism 5 (nonlinear curve ﬁt of Y = 100/(1 + 10((Log10IC50-X)*HillSlope)))	Download PDF
KKU-M055	11.59 µM	WST-1 assay; GraphPad Prism 5 (nonlinear curve ﬁt of Y = 100/(1 + 10((Log10IC50-X)*HillSlope)))	Download PDF
KKU-M156	6.25 µM	WST-1 assay; GraphPad Prism 5 (nonlinear curve ﬁt of Y = 100/(1 + 10((Log10IC50-X)*HillSlope)))	Download PDF
Ba/F3 cells expressing BCR-ABL1 Y253F	200-250 nM	XTT assay	Download PDF
Ba/F3 cells expressing BCR-ABL1 F317L	200-250 nM	XTT assay	Download PDF
BV-173	50 nM	XTT assay	Download PDF
SupB15	150 nM	XTT assay	Download PDF
PH PD-LTCs	50-100 nM	XTT assay	Download PDF
BV PD-LTCs	50-100 nM	XTT assay	Download PDF
KÖ PD-LTCs	50-100 nM	XTT assay	Download PDF
H3122	0.05 μM	MTT assay	PMC8636216
H2228	0.08 μM	MTT assay	PMC8636216
A549/R	30 –45μM	Cell Titer-Glo analysis reagent, microplate photoluminescence instrument, nonlinear regression of log to the response	Download PDF
H1299	15 –20μM	Cell Titer-Glo analysis reagent, microplate photoluminescence instrument, nonlinear regression of log to the response	Download PDF
3PAR	0.0080 µM	CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay (MTS analysis)	PMC7653150
8PAR	0.016 µM	CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay (MTS analysis)	PMC7653150
12PAR	0.015 µM	CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay (MTS analysis)	PMC7653150
3CRR	>1 µM	CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay (MTS analysis)	PMC7653150
8CRR	>1 µM	CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay (MTS analysis)	PMC7653150
12CRR	>1 µM	CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay (MTS analysis)	PMC7653150
BxPC3	15.60 µM	Cell viability studies	Download PDF
Capan 2	12.08 µM	Cell viability studies	Download PDF
Suit 2	18.97 µM	Cell viability studies	Download PDF
Suit 2	13.44 µM	Cell viability studies	Download PDF
Suit 2	11.46 µM	Cell viability studies	Download PDF
MIA PaCa-2	10.14 µM	Cell viability studies	Download PDF
MIA PaCa-2	6.41 µM	Cell viability studies	Download PDF
HNE-1 cells	0.79 ±0.21 µmol/L	Cell Counting Kit-8 (CCK-8)	PMC4555972
H1975	4 ± 0.1 µM	Sulforhodamine B assay	Download PDF
EBC-1	50 ± 2 nM	Sulforhodamine B assay	Download PDF
HCC827GR5	>5 µM	Sulforhodamine B assay	Download PDF
LoVo	13.6 ± 1.4 nM	clonogenic survival assay	PMC6226619
HCT116	315 ± 16 nM	clonogenic survival assay	PMC6226619
DLD1	217 ± 11 nM	clonogenic survival assay	PMC6226619
HCC78	100 nM	assay	Download PDF
JFCR-165	30 nM	assay	Download PDF
JFCR-168	30 nM	assay	Download PDF
MGH193-1B	30 nM	assay	Download PDF
KM12	>10000 nM	assay	Download PDF
MGH021-2 culture (CK8/18-positive cells)	1,300 nM	Immunoﬂuorescence-based cell scoring (CK8/18-positive cell count)	Download PDF
MGH051-1 culture (CK8/18-positive cells)	60 nM	Immunoﬂuorescence-based cell scoring (CK8/18-positive cell count)	Download PDF
MGH092-1 culture (CK8/18-positive cells)	166 nM	Immunoﬂuorescence-based cell scoring (CK8/18-positive cell count)	Download PDF
COS	340 nM	MTS assay, Nonlinear regression using log-transformed values.	PMC4562978
033 T PDX cells	691 nmol/L	Cell viability analyzed using an adenosine triphosphate monitoring system based on firefly luciferase (ATPlite 1step); IC50 values calculated using S+ Chip Analyzer.	Download PDF
PC-9 rociletinib-resistant	1881 ± 159 nM	assay	Download PDF
NCI-H460	14.29 μM	MTT assay, Logit method	Download PDF
A549	11.25 μM	MTT assay, Logit method	Download PDF
IB112	8.7 mM	Luminescent Cell Viability Assay	PMC5729471
IB118	8.8 mM	Luminescent Cell Viability Assay	PMC5729471
IB133	7.5 mM	Luminescent Cell Viability Assay	PMC5729471
IB134	3.5 mM	Luminescent Cell Viability Assay	PMC5729471
IB136	7.0 mM	Luminescent Cell Viability Assay	PMC5729471
SK-LMS-1	4.2 mM	Luminescent Cell Viability Assay	PMC5729471
RH4	1.5 μM	MTT assay	Download PDF
RH30	1.5 μM	MTT assay	Download PDF
HCN4-expressing HEK-293 cells	1.4 ± 0.3 μmol/L	Whole-cell patch clamp technique	Download PDF
SK-N-BE(2)	3.80 μM	Alamar Blue assay	PMC5491461
SH-SY5Y	0.71 μM	Alamar Blue assay	PMC5491461
KELLY	0.83 μM	Alamar Blue assay	PMC5491461
CHLA-20	1.16 μM	Alamar Blue assay	PMC5491461
SK-N-AS	8.65 μM	Alamar Blue assay	PMC5491461
MKN45, Hs746t	< 57 nM	publicly available data from the Cancer Cell Line Encyclopedia (CCLE) project	Download PDF
SH-SY5Y	480.6 nM	Cell number estimated after cell fixation with 10% trichloroacetic acid and staining with sulforhodamine B in 1% acetic acid, calculated by nonlinear regression analysis using GRAPHPAD PRISM.	Download PDF
LAN-1	236.6 nM	Cell number estimated after cell fixation with 10% trichloroacetic acid and staining with sulforhodamine B in 1% acetic acid, calculated by nonlinear regression analysis using GRAPHPAD PRISM.	Download PDF
SK-N-SH	3059.5 nM	Cell number estimated after cell fixation with 10% trichloroacetic acid and staining with sulforhodamine B in 1% acetic acid, calculated by nonlinear regression analysis using GRAPHPAD PRISM.	Download PDF
Kelly	432.4 nM	Cell number estimated after cell fixation with 10% trichloroacetic acid and staining with sulforhodamine B in 1% acetic acid, calculated by nonlinear regression analysis using GRAPHPAD PRISM.	Download PDF
CLB-GA	167.6 nM	Cell number estimated after cell fixation with 10% trichloroacetic acid and staining with sulforhodamine B in 1% acetic acid, calculated by nonlinear regression analysis using GRAPHPAD PRISM.	Download PDF
LAN-5	132.0 nM	Cell number estimated after cell fixation with 10% trichloroacetic acid and staining with sulforhodamine B in 1% acetic acid, calculated by nonlinear regression analysis using GRAPHPAD PRISM.	Download PDF
IMR-32	511.7 nM	Cell number estimated after cell fixation with 10% trichloroacetic acid and staining with sulforhodamine B in 1% acetic acid, calculated by nonlinear regression analysis using GRAPHPAD PRISM.	Download PDF
A549 cells	(3.345±1.625) μmol/L	MTT assay, absorbance at 490 nm, SPSS 17.0 software for calculation.	Download PDF
HCC827	6.05 ± 0.11 µmol/L	Sulforhodamine B (SRB) assay, analyzed with WinNonlin software.	PMC5143436
EBC-1	0.054 ± 0.002 µmol/L	Sulforhodamine B (SRB) assay, analyzed with WinNonlin software.	PMC5143436
LUDLU-1	8.12 ± 0.28 µmol/L	Sulforhodamine B (SRB) assay, analyzed with WinNonlin software.	PMC5143436
Karpas 299	54.1 nM	Cell Counting Kit-8 (CCK-8)	PMC5912135
SU-DHL-1	53.4 nM	Cell Counting Kit-8 (CCK-8)	PMC5912135
OVCAR-3	0.21 μM	colorimetric assay using SRB	Download PDF
IGROV-1	0.26 μM	colorimetric assay using SRB	Download PDF
A2780	0.71 μM	colorimetric assay using SRB	Download PDF
SKOV-3	3.29 μM	colorimetric assay using SRB	Download PDF
EFO-21	4.37 μM	colorimetric assay using SRB	Download PDF
NB1643	0.5 μM	Dose-response curve analysis for cell viability	Download PDF
NB1	0.3 μM	Dose-response curve analysis for cell viability	Download PDF
SH-SY5Y	1 μM	Dose-response curve analysis for cell viability	Download PDF
NPM-ALK positive ALCL cell lines	24 nmol/L	cell-based selectivity assays	Download PDF
FC-IBC01	0.89 μM	ProMegaCellTiter Cell Proliferation Assay	Download PDF
Mary-X	0.87 μM	ProMegaCellTiter Cell Proliferation Assay	Download PDF
SUM149	0.77 μM	ProMegaCellTiter Cell Proliferation Assay	Download PDF
MDA-IBC-3	1.98 μM	ProMegaCellTiter Cell Proliferation Assay	Download PDF
SUM190	5.20 μM	ProMegaCellTiter Cell Proliferation Assay	Download PDF
KPL-4	6.45 μM	ProMegaCellTiter Cell Proliferation Assay	Download PDF
IMR-32 wt ALK	0.74 μM	ProMegaCellTiter Cell Proliferation Assay	Download PDF
SNU-620	16.4 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
SNU-5	5.5 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
Hs 746T	12.6 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
GTL16	29.8 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
SNU-638	12.2 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
PAMC82	8254 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
IM95m	133 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
NUGC-3	5785 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
OCUM-1	974 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
SNU-601	2762 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
NUGC-4	4575 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
NCI-N87	5374 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
SNU-216	5386 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
MKN1	2090 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
SNU-1	666 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
MKN74	4754 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
HGC-27	6942 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
3132/87	1717 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
AGS	1430 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
SNU-484	2400 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
AZ-521	1825 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
KATO III	3122 nM	Standard metabolism-based anti-proliferative assays	PMC3950883
SNU-16	973 nM	Standard metabolism-based anti-proliferative assays	PMC3950883

Check the PF-02341066 (Crizotinib) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Size	Price	Qty
5mg	USD 147
50mg	USD 570
200mg	USD 970

Cell-free Assays (IC50/Ki) for PF-02341066 (Crizotinib)

Cell-based Assay (IC50) for PF-02341066 (Crizotinib)

Jump to

Cell-free Assays (IC₅₀/K_i) for PF-02341066 (Crizotinib)

Cell-based Assay (IC₅₀) for PF-02341066 (Crizotinib)