CAS No. 3778-73-2

Batch: Purity:99.24
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Ifosfamide Chemical Structure
Chemical Structure
Check the Ifosfamide product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name N,3-bis(2-chloroethyl)-2-oxo-1,3,2λ5-oxazaphosphinan-2-amine
CAS Number 3778-73-2
Molecular Formula

C7H15Cl2N2O2P
Molecular Weight (MW) 261.09
SMILES C1CN(P(=O)(OC1)NCCCl)CCCl
InChIKey HOMGKSMUEGBAAB-UHFFFAOYSA-N

Ordering Information

Biological Activity

Ifosfamide is a synthetic nitrogen mustard alkylating agent and DNA/RNA synthesis inhibitor. Upon metabolic activation, it generates reactive alkylating metabolites that interact with cellular macromolecules, causing interstrand DNA cross-linking. This DNA damage interrupts nucleic acid replication and RNA transcription, subsequently triggering cell cycle arrest, inhibition of cellular proliferation, and activation of apoptotic signaling pathways in cancer cells. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 52 mg/mL (199.16 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 52 mg/mL
Ethanol 52 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.