CAS No. 58-93-5

Batch: Purity:99.86
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Hydrochlorothiazide Chemical Structure
Chemical Structure
Check the Hydrochlorothiazide product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 6-chloro-1,1-dioxo-3,4-dihydro-2H-1λ6,2,4-benzothiadiazine-7-sulfonamide
CAS Number 58-93-5
Molecular Formula

C7H8ClN3O4S2
Molecular Weight (MW) 297.74
SMILES C1NC2=CC(=C(C=C2S(=O)(=O)N1)S(=O)(=O)N)Cl
InChIKey JZUFKLXOESDKRF-UHFFFAOYSA-N

Biological Activity

Hydrochlorothiazide is a thiazide class diuretic compound that acts as an inhibitor of the renal sodium-chloride cotransporter (NCC/SLC12A3). Inhibition of this cotransporter impairs sodium and chloride reabsorption in the distal convoluted tubule of the nephron. This blockade enhances renal excretion of sodium, chloride, and water, thereby modulating systemic electrolyte balance and renal fluid transport pathways. [1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
0.5MNAOH 3 mg/mL
DMSO 0 mg/mL (0.0 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Water 0 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
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Reference

[1] GambaG, et al. Proc. Natl. Acad. Sci. U.S.A. 1993;90(7):2749-2753.

[2] Gamba G. Physiol Rev. 2005;85(2):423-93.

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Note: Technical data last updated: Sep 1, 2026.