CAS No. 54-31-9

Batch: Purity:99.96
Furosemide Chemical Structure
Chemical Structure
Check the Furosemide product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 4-chloro-2-(furan-2-ylmethylamino)-5-sulfamoylbenzoic acid
CAS Number 54-31-9
Molecular Formula

C12H11ClN2O5S

Molecular Weight (MW) 330.74
SMILES C1=COC(=C1)CNC2=CC(=C(C=C2C(=O)O)S(=O)(=O)N)Cl
InChIKey ZZUFCTLCJUWOSV-UHFFFAOYSA-N

Ordering Information

Biological Activity

Furosemide is a potent inhibitor of the Na-K-2Cl symporter (NKCC2). It acts primarily in the thick ascending limb of the loop of Henle to block ion transport, functioning independently of carbonic anhydrase or aldosterone inhibition. By abolishing the corticomedullary osmotic gradient, furosemide effectively blocks both positive and negative free water clearance, leading to reduced solute reabsorption and altered renal tubular electrolyte transport.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 66 mg/mL (199.55 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.