research use only

Box5

Cat.No.P1216

Box5, a Wnt5a-derived N-butyloxycarbonyl hexapeptide (t-Boc-Met-Asp-Gly-Cys-Glu-Leu), acts as an Wnt5a antagonist, inhibits the basal migration and invasion of Wnt5a-expressing HTB63 melanoma cells, antagonizes the effects of Wnt5a on melanoma cell migration and invasion by directly inhibiting Wnt5a-induced PKC and Ca(2+) signaling.

Amino-acid Sequence

CAS No. 881188-48-3

Selleck's Box5 Has Been Cited by 4 Publications

Quality Control

Batch: P121601 DMSO] 100 mg/mL] false] Ethanol] 100 mg/mL] false] Water] 2 mg/mL] false Purity: 98.20%

98.20

Chemical Information & Solubility

Storage (From the date of receipt)	3 years-20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
			1 month-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL (149.97 mM)；
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 100 mg/mL

Water : 2 mg/mL

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.					In vivo Formulation Calculator
	Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.	In vivo Formulation Calculator
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	5.000mg/ml (7.50mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	0.830mg/ml (1.24mM)	Taking the 1 mL working solution as an example, add 50 μL of 16.6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

Biological Activity

Description	Box5, a Wnt5a-derived N-butyloxycarbonyl hexapeptide (t-Boc-Met-Asp-Gly-Cys-Glu-Leu), acts as an Wnt5a antagonist, inhibits the basal migration and invasion of Wnt5a-expressing HTB63 melanoma cells, antagonizes the effects of Wnt5a on melanoma cell migration and invasion by directly inhibiting Wnt5a-induced PKC and Ca(2+) signaling.
Targets/IC₅₀/Ki	Wnt5a

References
https://pubmed.ncbi.nlm.nih.gov/19901340/

References

https://pubmed.ncbi.nlm.nih.gov/19901340/

Product Information

Molecular Formula	C₂₅H₄₂N₆O₁₁S₂
Molecular Weight	666.76
CAS No.	881188-48-3
Appearance	White Powder
Peptide Content (by %N)	≥80%
Single Impurity (by HPLC)	≤1.0%
Assay (By Anhydrous, Acetic Acid-free)	95.0~105.0%
MS	Consistent
Packing	plastic vial(dedicated for peptide packing) or glass vial, quantity according to customer's detail requirement.
Storage (From the date of receipt)	3 years-20°C powder
Shipping	Room temperature shipping(Stability testing shows this product can be shipped without any cooling measures.)