research use only

Apamin

Cat.No.P1234

Apamin, an 18 amino acid peptide neurotoxin present in apitoxin (bee venom), has been shown to have anti-inflammatory and anti-fibrotic properties. It is known to be a highly selective blocker of Ca²⁺-activated K⁺ (SK) channels and then lessen the expression of α-SMA in the TGF-β1-induced HSC-T6 cells.

Amino-acid Sequence

CAS No. 24345-16-2

Quality Control

Batch: Purity: 99.93%

99.93

Chemical Information & Solubility

Storage (From the date of receipt)	3 years-20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
			1 month-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL (49.32 mM)；
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 100 mg/mL

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.					In vivo Formulation Calculator
	Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.	In vivo Formulation Calculator
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	5.000mg/ml (2.47mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	0.830mg/ml (0.41mM)	Taking the 1 mL working solution as an example, add 50 μL of 16.6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

Biological Activity

Description	Apamin, an 18 amino acid peptide neurotoxin present in apitoxin (bee venom), has been shown to have anti-inflammatory and anti-fibrotic properties. It is known to be a highly selective blocker of Ca²⁺-activated K⁺ (SK) channels and then lessen the expression of α-SMA in the TGF-β1-induced HSC-T6 cells.
Targets/IC₅₀/Ki	Potassium channel

References
https://pubmed.ncbi.nlm.nih.gov/28405682/

References

https://pubmed.ncbi.nlm.nih.gov/28405682/

Product Information

Molecular Formula	C₇₉H₁₃₁N₃₁O₂₄S₄
Molecular Weight	2027.35
CAS No.	24345-16-2
Appearance	White Powder
Peptide Content (by %N)	≥80%
Single Impurity (by HPLC)	≤1.0%
Assay (By Anhydrous, Acetic Acid-free)	95.0~105.0%
MS	Consistent
Packing	plastic vial(dedicated for peptide packing) or glass vial, quantity according to customer's detail requirement.
Storage (From the date of receipt)	3 years -20°C powder； 1 years -80°C in solvent
Shipping	Room temperature shipping(Stability testing shows this product can be shipped without any cooling measures.)