Sodium Nitrite

Catalog No.S4074

Sodium Nitrite Chemical Structure

Molecular Weight(MW): 69

Sodium nitrite is a myeloperoxidase inhibitor with IC50 of 1.3 μM.

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Biological Activity

Description Sodium nitrite is a myeloperoxidase inhibitor with IC50 of 1.3 μM.
Targets
myeloperoxidase [1]
1.3 μM(Kd)
In vitro

Sodium nitrite is well known for its role in inhibiting the growth of Clostridium botulinum by inhibiting iron-sulfur clusters essential to energy metabolism. Sodium nitrite slows chlorination by univalently reducing myeloperoxidase to an inactive form and as a consequence is oxidized to nitrogen dioxide. Myeloperoxidase oxidizes free tyrosine to tyrosyl radicals that exchange with tyrosyl residues in peptides. These peptide radicals then couple with nitrogen dioxide to form 3-nitrotyrosyl residues. With neutrophils, myeloperoxidase-dependent nitration required a high concentration of nitrite (1 mM), is doubled by tyrosine, and increases 4-fold by superoxide dismutase. Superoxide is likely to inhibit nitration by reacting with nitrogen dioxide and/or tyrosyl radicals. [1] Sodium nitrite results in intoxication of white mice with decrease of red cell superoxide dismutase (SOD) and catalase activity. The total activity of glucose-6-phosphate dehydrogenase and dehydrogenase of 6-phosphogluconate as well as the activity of glutathione reductase are higher in the group of mice that receive sodium nitrite in comparison with the control group. [3]

In vivo Peak plasma levels of nitrite are achieved in both sexes approximately 30 min after oral exposure. The model predicts that 10% of the hemoglobin is oxidized to the ferric form after oral doses of 15.9 mg/kg in male rats and 11.0 mg/kg in female rats and after intravenous doses of 8.9 and 7.1 mg/kg in male and female rats, respectively. The t1/2 for recovery from methemoglobinemia is 60 to 120 min depending on dose and route of administration. Replacement of the Vmax of methemoglobin reductase with a value representative of humans predicted a 10% methemoglobinemia following an intravenous dose of 5.8 mg/kg, in close agreement with an observed value of 5.7 mg/kg for humans. [2]

Protocol

Kinase Assay:

[1]

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H2O2 uptake assay:

Reactions are started by adding 10 nM myeloperoxidase to 30 μM H 2 O 2 and varying concentrations of nitrite, in 100 mM phosphate buffer, pH 7.4, containing 20 μM DTPA. Steady state rates of H 2 O 2 loss at 21°C are calculated over the first minute. Data are means and ranges of at least duplicate experiments.
Cell Research:

[4]

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  • Cell lines: Jurkat cells
  • Concentrations: ~25 μM
  • Incubation Time: 36 h
  • Method:

    cell density


    (Only for Reference)
Animal Research:

[2]

+ Expand
  • Animal Models: Twelve-week old Fischer 344 rats
  • Formulation: deionized water
  • Dosages: 20 mg/kg
  • Administration: tail vein injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (188.4 mM)
Water 13 mg/mL warmed (188.4 mM)
Ethanol Insoluble
In vivo Add solvents individually and in order:
30% propylene glycol, 5% Tween 80, 65% D5W
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 69
Formula

HNO2.Na

CAS No. 7632-00-0
Storage powder
Synonyms N/A

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01316796 Completed Sickle Cell Anemia|Sickle Cell Disease|Chronic Hemolytic Disorders National Heart, Lung, and Blood Institute (NHLBI)|National Institutes of Health Clinical Center (CC) March 9, 2011 Phase 1
NCT00105222 Terminated Healthy|Healthy Volunteer|HV National Heart, Lung, and Blood Institute (NHLBI)|National Institutes of Health Clinical Center (CC) March 8, 2005 Phase 1
NCT00103025 Completed Healthy National Institute of Neurological Disorders and Stroke (NINDS)|National Institutes of Health Clinical Center (CC) February 4, 2005 Phase 1
NCT00098072 Completed Hypertension, Pulmonary National Institutes of Health Clinical Center (CC) November 30, 2004 Phase 1
NCT03015402 Not yet recruiting Pulmonary Hypertension Secondary|Heart Failure University of Pittsburgh|National Heart, Lung, and Blood Institute (NHLBI) June 2017 Phase 2
NCT02517697 Not yet recruiting Metabolic Syndrome|Hypertension University of Pittsburgh June 2017 Phase 2

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID