Oligomycin A

Catalog No.S1478 Synonyms: MCH 32

Oligomycin A Chemical Structure

Molecular Weight(MW): 791.06

Oligomycin A is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.

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In DMSO USD 390 In stock
USD 70 In stock
USD 270 In stock
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  • (F) Detection of expression of SDHA and TOMM20 by immunoblotting in wild-type and Atg7-/- K562 cells with indicated drugs. The expression levels were quantified with number indicated. (G) Quantification analysis of mitochondrial mass in wild-type and Atg7-/- K562 cells when cultured with oligomycin(100 ng/ml), antimycin A(50 μM) and Baf-A1(10 nM).

    Sci Rep, 2016, 6:24641.. Oligomycin A purchased from Selleck.

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Biological Activity

Description Oligomycin A is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.
Targets
ATP synthase [1]
(Cell-free assay)
In vitro

Oligomycin A is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.Oligomycin A inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. The inhibition of ATP synthesis by oligomycin A will significantly reduce electron flow through the electron transport chain; however, electron flow is not stopped completely due to a process known as proton leak or mitochondrial uncoupling. [1] In a group of cancer cells, Oligomycin at 100 ng/ml completely inhibits oxidative phosphorylation activity in 1 h and induces various levels of glycolysis gains by 6 h. [2] Oligomycin, inhibitor of the F0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis. [3] Oligomycin inhibits mitochondrial respiration in melanoma, sensitizes melanoma cells to therapy, blocks the emergence of the slow-cycling, long-term tumor-maintaining melanoma cells. [4]

Protocol

Cell Research:[2]
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  • Cell lines: H1229
  • Concentrations: 100 ng/ml
  • Incubation Time: 1 h
  • Method: ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 10 mg/mL (12.64 mM)
Water slightly soluble or insoluble
Ethanol slightly soluble or insoluble
In vivo Add solvents individually and in order:
30% PEG400+0.5% Tween80+5% propylene glycol
15 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 791.06
Formula

C45H74O11

CAS No. 579-13-5
Storage powder
in solvent
Synonyms MCH 32

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID