CAS No. 1391737-01-1
Batch:
Purity:99.82
Chemical Structure
Check the
VU0463271
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | N-cyclopropyl-N-(4-methyl-1,3-thiazol-2-yl)-2-(6-phenylpyridazin-3-yl)sulfanylacetamide | |
|---|---|---|
| CAS Number | 1391737-01-1 | |
| Molecular Formula |
C19H18N4OS2 |
|
| Molecular Weight (MW) | 382.51 | |
| InChIKey | DPONSKCACOZTGN-UHFFFAOYSA-N |
Biological Activity
VU0463271 is a potent antagonist of neuronal-specific potassium-chloride cotransporter 2 (KCC2) with an IC50 of 61 nM, demonstrating over 100-fold selectivity over Na-K-2Cl cotransporter 1 (NKCC1). Blockade of KCC2-mediated chloride extrusion disrupts intracellular chloride homeostasis and impairs GABAergic inhibitory neurotransmission. This disinhibition causes a depolarizing shift in GABA reversal potential, leading to enhanced neuronal spiking and network hyperexcitability. [1][2]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
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Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
26 mg/mL
(67.97 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 2 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.