CAS No. 224785-91-5

Batch: Purity:99.53
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Vardenafil hydrochloride Chemical Structure
Chemical Structure
Check the Vardenafil hydrochloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 2-[2-ethoxy-5-(4-ethylpiperazin-1-yl)sulfonylphenyl]-5-methyl-7-propyl-3H-imidazo[5,1-f][1,2,4]triazin-4-one;hydrochloride
CAS Number 224785-91-5
Molecular Formula

C23H33ClN6O4S
Molecular Weight (MW) 525.06
SMILES Cl.CCCC1=NC(=C2[N]1NC(=NC2=O)C3=C(OCC)C=CC(=C3)[S](=O)(=O)N4CCN(CC)CC4)C
InChIKey XCMULUAPJXCOHI-UHFFFAOYSA-N

Biological Activity

Vardenafil hydrochloride is a selective, orally active phosphodiesterase inhibitor of PDE5, PDE6, and PDE1. By inhibiting PDE5, it prevents the hydrolysis of cyclic guanosine monophosphate (cGMP), thereby increasing intracellular cGMP levels. This elevation activates downstream cGMP/PKG signaling, promoting cGMP-dependent protein kinase activity and modulating vascular smooth muscle relaxation and cellular responses.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (190.45 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 100 mg/mL
Ethanol 20 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.