Clinical Trials

Numerous clinical trials evaluate therapeutic applications across diverse inflammatory, autoimmune, and perioperative conditions, including ulcerative colitis, rheumatoid arthritis, systemic lupus erythematosus, spondyloarthropathy, ocular inflammatory diseases, alopecia, and opioid-free anesthesia care. Ranging from Phase 1/2 evaluations to Phase 4 trials and observational studies, these investigations are led by industry sponsors like Pfizer alongside academic medical centers. Their recruitment statuses range from completed to actively recruiting or unknown.

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05577962 RECRUITING
Opioid-free Anesthesia
Beijing Friendship Hospital
2024-08-01 PHASE4
NCT07406204 NOT_YET_RECRUITING
Alopecia Areata; Alopecia Totalis (AT); Alopecia Universalis
Hayat Abad Medical Complex, Peshawar
2026-02-15 PHASE4
NCT04485325 COMPLETED
Rheumatic Arthritis
Dr. Frank Behrens
2019-11-04 PHASE4
NCT03755466 UNKNOWN
Rheumatoid Arthritis
Shinshu University
2018-11-21 PHASE2
NCT05133297 COMPLETED
Rheumatoid Arthritis
Hangzhou Highlightll Pharmaceutical Co., Ltd
2022-02-16 PHASE2
NCT05728008 Completed
Ulcerative Colitis
IRCCS San Raffaele
2022-04-05 --
NCT04721808 Completed
Arthritis Rheumatoid
Pfizer
2021-01-22 --
NCT03580343 UNKNOWN
Uveitis; Scleritis
Washington University School of Medicine
2019-04-04 PHASE2
NCT03288324 Completed
Cutaneous Lupus|Systemic Lupus Erythematosus
Children''s Hospital Medical Center Cincinnati|Pfizer
2017-08-23 Phase 1|Phase 2
NCT01741493 COMPLETED
Rheumatoid Arthritis
AbbVie (prior sponsor, Abbott)
2012-11 PHASE1

(data from https://clinicaltrials.gov, updated on 2026-08-05)

Check the TOFA product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Compliance for Clinical Use

Mechanism and Biochemical Profile

TOFA intracellularly converts into TOFyl-CoA to allosterically inhibit acetyl-CoA carboxylase-α (ACCA), thereby blocking downstream de novo fatty acid synthesis and disrupting crucial lipid metabolic pathways. This disruption leads to dose-dependent cell death, offering clinical relevance in modulating metabolic and inflammatory processes associated with conditions such as ulcerative colitis and rheumatoid arthritis.

Appendix RUO and cGMP Quality Standards

Quality Dimension RUO (Research Use Only) cGMP (Current Good Manufacturing Practice)
Clinical Applicability Prohibited in human clinical trials or medical diagnostics. Mandatory for human clinical trials (Phase I–III) and therapies.
Regulatory Status Non-regulated grade; exempt from drug manufacturing laws. Legally enforced by health authorities (e.g., FDA, EMA, NMPA).
Facility Environment Unclassified analytical or research laboratories. Validated Cleanrooms (ISO Class 5–8) with continuous monitoring.
Quality Control Basic purity and activity testing. Rigorous safety release testing (Sterility, Endotoxin, Mycoplasma).
Process Validation Basic equipment calibration; no process validation required. Full qualification (IQ/OQ/PQ) and complete batch records.
Quality Assurance Vendor self-declared without required formal QMS. Mandatory QA/QC unit, Change Control, CAPA, and vendor audits.
Regulatory Impact High risk of IND rejection if used as a critical raw material. Required for IND/NDA filings, supported by Drug Master Files (DMF).

Footnotes

Regulatory Note: Governed by FDA (21 CFR Parts 210/211/312), EMA (EudraLex Vol 4), ICH Guidelines (E6/Q7/Q9/Q10), and compendial standards (USP <71>/<85>/<1043>).

Note: Technical data last updated: Sep 1, 2026.