CAS No. 50-89-5
Batch:
Purity:99.98
Chemical Structure
Check the
Thymidine
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione | |
|---|---|---|
| CAS Number | 50-89-5 | |
| Molecular Formula |
C10H14N2O5 |
|
| Molecular Weight (MW) | 242.23 | |
| SMILES | CC1=CN(C(=O)NC1=O)C2CC(C(O2)CO)O | |
| InChIKey | IQFYYKKMVGJFEH-XLPZGREQSA-N |
Biological Activity
Thymidine is a pyrimidine nucleoside involved in nucleic acid synthesis and cell cycle regulation. High exogenous concentrations of thymidine disrupt balanced intracellular deoxyribonucleoside triphosphate pools, leading to the inhibition of cellular DNA replication. This reversible blockade of DNA synthesis is widely employed in cell culture models to arrest populations at the G1/S transition, facilitating synchronized cell cycle progression and functional genomic analysis. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
48 mg/mL
(198.15 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 48 mg/mL |
| Ethanol | 9 mg/mL |
| DMSO |
48 mg/mL
(198.15 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 48 mg/mL |
| Ethanol | 6 mg/mL |
| DMSO |
48 mg/mL
(198.15 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 48 mg/mL |
| Ethanol | 2 mg/mL |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Pre-aliquoted for Longevity: Solid-state aliquots offer longer shelf life compared to stock solutions.
Looking for a specific aliquot size? Contact your local sales representative or orders@selleckchem.com.
Reference
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Note: Technical data last updated: Sep 1, 2026.