CAS No. 298-93-1
Batch:
Purity:99.86
Chemical Structure
Check the
Thiazolyl Blue
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 2-(3,5-diphenyltetrazol-2-ium-2-yl)-4,5-dimethyl-1,3-thiazole bromide | |
|---|---|---|
| CAS Number | 298-93-1 | |
| Molecular Formula |
C18H16BrN5S |
|
| Molecular Weight (MW) | 414.32 | |
| SMILES | CC1=C(SC(=N1)[N+]2=NC(=NN2C3=CC=CC=C3)C4=CC=CC=C4)C.[Br-] | |
| InChIKey | AZKSAVLVSZKNRD-UHFFFAOYSA-M |
Biological Activity
Thiazolyl Blue (MTT) is a cell-permeable colorimetric tetrazolium dye used as an indicator of cellular metabolic activity and viability. In viable cells, NAD(P)H-dependent cellular oxidoreductase enzymes reduce yellow MTT into insoluble purple formazan crystals. This enzymatic reduction directly correlates with cellular metabolic state and viable cell count, providing a quantitative readout for evaluating cell proliferation, cytotoxicity, and apoptosis across diverse cell-based assays. [1][2]
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
21 mg/mL
(50.68 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 7 mg/mL |
| Water | 2 mg/mL |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.