CAS No. 64-75-5
Batch:
Purity:99.17
Chemical Structure
Check the
Tetracycline HCl
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | (4S,4aS,5aS,6S,12aR)-4-(dimethylamino)-1,6,10,11,12a-pentahydroxy-6-methyl-3,12-dioxo-4,4a,5,5a-tetrahydrotetracene-2-carboxamide;hydrochloride | |
|---|---|---|
| CAS Number | 64-75-5 | |
| Molecular Formula |
C22H24N2O8.HCl |
|
| Molecular Weight (MW) | 480.9 | |
| SMILES | CC1(C2CC3C(C(=O)C(=C(C3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O.Cl | |
| InChIKey | YCIHPQHVWDULOY-FMZCEJRJSA-N |
Biological Activity
Tetracycline HCl is a broad-spectrum polyketide antibiotic that targets the microbial ribosome. By binding specifically to the 30S ribosomal subunit, tetracycline blocks the attachment of charged aminoacyl-tRNA to the ribosomal A site. This inhibition prevents the introduction of new amino acids into the nascent peptide chain, thereby disrupting bacterial protein synthesis and inhibiting microbial cell proliferation. [1][2]
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
96 mg/mL
(199.62 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 80 mg/mL |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.