CAS No. 465-39-4

Batch: Purity:99.96
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Resibufogenin Chemical Structure
Chemical Structure
Check the Resibufogenin product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 5-[(1R,2S,4R,6R,7R,10S,11S,14S,16R)-14-hydroxy-7,11-dimethyl-3-oxapentacyclo[8.8.0.02,4.02,7.011,16]octadecan-6-yl]pyran-2-one
CAS Number 465-39-4
Molecular Formula

C24H32O4
Molecular Weight (MW) 384.51
SMILES CC12CCC(O)CC1CCC3C2CCC4(C)C(CC5OC345)C6=COC(=O)C=C6
InChIKey ATLJNLYIJOCWJE-CWMZOUAVSA-N

Ordering Information

Biological Activity

Resibufogenin is a bioactive bufadienolide component of huachansu with potent anticancer activity. It triggers necroptosis by upregulating receptor-interacting protein kinase 3 (RIP3) and promoting the phosphorylation of mixed lineage kinase domain-like protein (MLKL) at Ser358. Furthermore, resibufogenin induces reactive oxygen species (ROS) accumulation and activates caspase-3 and caspase-8 to promote apoptosis, while downregulating PI3K, p-AKT, p-GSK3β, cyclin D1, and β-catenin signaling pathways. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 77 mg/mL (200.25 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 77 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.