CAS No. 220904-99-4
Batch:
Purity:99.80
Chemical Structure
Check the
Raf inhibitor 2
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | (3Z)-5-chloro-3-[(3,5-dibromo-4-hydroxyphenyl)methylidene]-1H-indol-2-one | |
|---|---|---|
| CAS Number | 220904-99-4 | |
| Molecular Formula |
C15H8Br2ClNO2 |
|
| Molecular Weight (MW) | 429.49 | |
| SMILES | OC1=C(Br)C=C(C=C1Br)\C=C2/C(=O)NC3=C2C=C(Cl)C=C3 | |
| InChIKey | MPIHOJMBMIMKAJ-KMKOMSMNSA-N |
Raf inhibitor 2 is a synthetic small-molecule inhibitor targeting Raf kinase. By inhibiting Raf kinase, it suppresses the RAF/MEK/ERK signaling pathway and attenuates downstream phosphorylation cascades. This disruption impairs signaling through the MAPK network, leading to inhibited cell proliferation, decreased cell survival, and promoted apoptosis in cancer research models reliant on dysregulated Raf signaling.
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Concentration
Volume
Mass
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Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
86 mg/mL
(200.23 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.