CAS No. 553-12-8

Batch: Purity:99.02
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Protoporphyrin IX (PPIX) Chemical Structure
Chemical Structure
Check the Protoporphyrin IX (PPIX) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 553-12-8
Molecular Formula

C34H34N4O4
Molecular Weight (MW) 562.66

Biological Activity

Protoporphyrin IX (PPIX) is a heterocyclic porphyrin and the final intermediate in the heme biosynthetic pathway. It functions as an endogenous activator of soluble guanylate cyclase (sGC) to stimulate cyclic GMP signaling. Additionally, light activation of PPIX generates reactive oxygen species (ROS), inducing mitochondrial dysfunction, oxidative stress, and apoptotic cell death in target cells. [1]

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (177.72 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Ignarro LJ, et al. Proc Natl Acad Sci U S A. 1982;79(9):2870-3.

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Note: Technical data last updated: Sep 1, 2026.