CAS No. 99-50-3

Batch: Purity:99.99
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Protocatechuic acid Chemical Structure
Chemical Structure
Check the Protocatechuic acid product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 3,4-dihydroxybenzoic acid
CAS Number 99-50-3
Molecular Formula

C7H6O4
Molecular Weight (MW) 154.12
SMILES C1=CC(=C(C=C1C(=O)O)O)O
InChIKey YQUVCSBJEUQKSH-UHFFFAOYSA-N

Biological Activity

Protocatechuic acid is a naturally occurring phenolic acid with potent antioxidant and anti-inflammatory activities. It modulates key signal transduction pathways, including the NF-kB and MAPK signaling cascades, thereby attenuating the expression of pro-inflammatory cytokines such as TNF-alpha, IL-1beta, and IL-6. Additionally, protocatechuic acid scavenges reactive oxygen species (ROS) and upregulates endogenous antioxidant defenses, exerting neuroprotective, cardioprotective, and chemopreventive phenotypes across various in vitro and in vivo models. [1]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 30 mg/mL (194.65 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Kakkar S, et al. ISRN Pharmacol. 2014;2014:952943.

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Note: Technical data last updated: Sep 1, 2026.