CAS No. 24939-03-5

Batch: Purity:99.91
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Poly(I:C) (Polyinosinic-Polycytidylic Acid) Chemical Structure
Chemical Structure
Check the Poly(I:C) (Polyinosinic-Polycytidylic Acid) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 24939-03-5
Molecular Formula

C19H27N7O16P2
Molecular Weight (MW) 671.40
SMILES NC1=NC(=O)N(C=C1)C2OC(CO[P;v5](O)(O)=O)C(O)C2O.OC3C(O)C(OC3CO[P;v5](O)(O)=O)[N]4C=NC5=C4NC=NC5=O

Biological Activity

Poly(I:C) (Polyinosinic-Polycytidylic Acid) is a synthetic double-stranded RNA immunostimulatory agent that acts as a Toll-like receptor 3 (TLR3) agonist. Engagement of TLR3 by Poly(I:C) triggers downstream signaling through the NF-kB and MAP kinase pathways. This activation promotes the cellular production of type I interferons and inflammatory cytokines, stimulating innate immune responses and driving dendritic cell maturation. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 50 mg/mL
DMSO Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Alexopoulou L, et al. Nature. 2001;413(6857):732-738.

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Note: Technical data last updated: Sep 1, 2026.