CAS No. 70132-50-2
Batch:
Purity:99.52
Chemical Structure
Check the
Pimonidazole
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | 1-(2-nitroimidazol-1-yl)-3-piperidin-1-ylpropan-2-ol | |
|---|---|---|
| CAS Number | 70132-50-2 | |
| Molecular Formula |
C11H18N4O3 |
|
| Molecular Weight (MW) | 254.29 | |
| SMILES | OC(CN1CCCCC1)C[N]2C=CN=C2[N+]([O-])=O | |
| InChIKey | WVWOOAYQYLJEFD-UHFFFAOYSA-N |
Pimonidazole is a 2-nitroimidazole compound that acts as a small-molecule probe and biomarker for hypoxia. Under hypoxic cellular conditions, pimonidazole undergoes reductive activation by intracellular reductases to generate reactive intermediates that covalently bind to protein thiol groups, forming stable adducts. This enables the evaluation of cellular oxygenation, identification of hypoxic microenvironments, and complementary study of cell proliferation in solid malignancies, including cervical carcinoma. [1]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
51 mg/mL
(200.55 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 51 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.