CAS No. 9008-97-3

Batch: Purity:99
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Phytohemagglutinin (PHA) Chemical Structure
Chemical Structure
Check the Phytohemagglutinin (PHA) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 2-(3,4-dimethoxyphenyl)-5,7-dimethoxy-3,4-dihydro-2H-chromen-4-ol
CAS Number 9008-97-3
Molecular Formula

N/A
Molecular Weight (MW) N/A
InChIKey APLKIOPSKVXHFK-UHFFFAOYSA-N

Ordering Information

Biological Activity

Phytohemagglutinin (PHA) is a plant-derived lectin glycoprotein from Phaseolus vulgaris that acts as a potent mitogen to stimulate lymphocyte proliferation and transform them into blast cells. Upon T-cell activation, it triggers signaling pathways that upregulate pro-apoptotic proteins, including Bax, leading to caspase-3 activation. In downstream immune responses, PHA suppresses humoral antibody production and enhances homograft survival by inhibiting immune rejection. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 100 mg/mL
DMSO Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.