CAS No. 6130-64-9

Batch: Purity:99.91
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Penicillin G Procaine Chemical Structure
Chemical Structure
Check the Penicillin G Procaine product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 6130-64-9
Molecular Formula

C16H18N2O4S.C13H20N2O2.H2O
Molecular Weight (MW) 588.72
SMILES O.CCN(CC)CCOC(=O)C1=CC=C(N)C=C1.CC2(C)SC3C(NC(=O)CC4=CC=CC=C4)C(=O)N3C2C(O)=O

Biological Activity

Penicillin G procaine is a beta-lactam antibiotic and a procaine salt form of benzylpenicillin. It acts by binding to penicillin-binding proteins (PBPs) involved in bacterial cell wall peptidoglycan synthesis. Inhibition of PBPs disrupts the cross-linking of the peptidoglycan network, leading to bacterial cell wall weakening, osmotic lysis, and bactericidal activity against susceptible Gram-positive pathogens. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (169.86 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 5 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Spratt BG. Proc Natl Acad Sci U S A. 1975;72(8):2999-3003.

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Note: Technical data last updated: Sep 1, 2026.