Pembrolizumab (anti-PD-1)

Catalog No.A2005 Synonyms: MK-3475, lambrolizumab Non-humanized mouse model applicable

For research use only. Not for use in humans.

Pembrolizumab (anti-PD-1) is a potent, highly selective, fully humanized immunoglobulin (Ig) G4-kappa monoclonal antibody against PD-1 with potential immune checkpoint inhibitory and antineoplastic activities. MW : 146.286 KD.

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5mg	USD 707	In stock
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Biological Activity

Description

Pembrolizumab (anti-PD-1) is a potent, highly selective, fully humanized immunoglobulin (Ig) G4-kappa monoclonal antibody against PD-1 with potential immune checkpoint inhibitory and antineoplastic activities. MW : 146.286 KD.

Targets

PD-1 ^[1]
(Cell-free assay)

In vivo

In addition to Melanoma and Lung carcinoma, pembrolizumab has also demonstrated efficacy in other advanced solid tumors and hematologic malignancies. Pembrolizumab had an acceptable side-effect profile and showed antitumor activity in patients with advanced non-small-cell lung cancer^[2].

Protocol

Cell Research:	+ Expand Objective: To examine the effect of high-dose steroid therapy on lymphocyte proliferation/functionality after stimulation with anti-CD3 in the presence of pembrolizumab Cells: Human PBMCs Concentrations: 5 µg/mL Incubation Time: -- Method: Isolated PBMCs were pre-treated with prednisone (1 nM - 1 mM) before anti-CD3 stimulation. Anti-CD3-stimulated cells were then incubated with pembrolizumab and their proliferative capacity and IL-2 secretion ability were measured by carboxyfluorescein succinimidyl ester (CFSE) staining and ELISA. Reference: http://ascopubs.org/doi/abs/10.1200/JCO.2017.35.15_suppl.e14587 Pembrolizumab can apply to mice, rat, peripheral blood and other related assays (Only for Reference)
Animal Research:	+ Expand Objective: To evaluate the pharmacokinetics and biodistribution of 89Zr-labeled Pembrolizumab Animal Models: Female ICR (CD-1) mice, Hsd: Sprague-Dawley outbred rats, NSG mice(humanized mice), hu-PBL-SCID mice(humanized mice) Formulation: -- Dosages: -- Administration: i.v. Reference: https://www.ncbi.nlm.nih.gov/pubmed/27493273 Pembrolizumab can apply to mice, rat, peripheral blood and other related assays (Only for Reference)

Cell Research:

+ Expand

Objective: To examine the effect of high-dose steroid therapy on lymphocyte proliferation/functionality after stimulation with anti-CD3 in the presence of pembrolizumab
Cells: Human PBMCs
Concentrations: 5 µg/mL
Incubation Time: --
Method: Isolated PBMCs were pre-treated with prednisone (1 nM - 1 mM) before anti-CD3 stimulation. Anti-CD3-stimulated cells were then incubated with pembrolizumab and their proliferative capacity and IL-2 secretion ability were measured by carboxyfluorescein succinimidyl ester (CFSE) staining and ELISA.
Reference: http://ascopubs.org/doi/abs/10.1200/JCO.2017.35.15_suppl.e14587

Pembrolizumab can apply to mice, rat, peripheral blood and other related assays (Only for Reference)

Animal Research:

+ Expand

Objective: To evaluate the pharmacokinetics and biodistribution of 89Zr-labeled Pembrolizumab
Animal Models: Female ICR (CD-1) mice, Hsd: Sprague-Dawley outbred rats, NSG mice(humanized mice), hu-PBL-SCID mice(humanized mice)
Formulation: --
Dosages: --
Administration: i.v.
Reference: https://www.ncbi.nlm.nih.gov/pubmed/27493273

Pembrolizumab can apply to mice, rat, peripheral blood and other related assays (Only for Reference)

References

Product Details

Formulation	PBS buffer, pH 7.2
Isotype	Human IgG4
Source	CHO cells
Storage	Store at -80°C and avoid freeze-thaw cycles.

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