Pembrolizumab (anti-PD-1)

Catalog No.A2005 Synonyms: MK-3475, lambrolizumab

For research use only.
Not for use in humans.

Pembrolizumab (anti-PD-1) is a potent, highly selective, fully humanized immunoglobulin (Ig) G4-kappa monoclonal antibody against PD-1 with potential immune checkpoint inhibitory and antineoplastic activities. MW : 146.286 KD.

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Cited by 3 publications

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Choose Selective PD-1/PD-L1 Inhibitors

Biological Activity

Description Pembrolizumab (anti-PD-1) is a potent, highly selective, fully humanized immunoglobulin (Ig) G4-kappa monoclonal antibody against PD-1 with potential immune checkpoint inhibitory and antineoplastic activities. MW : 146.286 KD.
Targets
PD-1 [1]
(Cell-free assay)
In vivo In addition to Melanoma and Lung carcinoma, pembrolizumab has also demonstrated efficacy in other advanced solid tumors and hematologic malignancies. Pembrolizumab had an acceptable side-effect profile and showed antitumor activity in patients with advanced non-small-cell lung cancer[2].

Protocol

Cell Research:
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  • Objective: To examine the effect of high-dose steroid therapy on lymphocyte proliferation/functionality after stimulation with anti-CD3 in the presence of pembrolizumab
    Cells: Human PBMCs
    Concentrations: 5 µg/mL
    Incubation Time: --
    Method: Isolated PBMCs were pre-treated with prednisone (1 nM - 1 mM) before anti-CD3 stimulation. Anti-CD3-stimulated cells were then incubated with pembrolizumab and their proliferative capacity and IL-2 secretion ability were measured by carboxyfluorescein succinimidyl ester (CFSE) staining and ELISA.
    Reference: http://ascopubs.org/doi/abs/10.1200/JCO.2017.35.15_suppl.e14587

    Pembrolizumab can apply to mice, rat, peripheral blood and other related assays (Only for Reference)

Animal Research:
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  • Objective: To evaluate the pharmacokinetics and biodistribution of 89Zr-labeled Pembrolizumab
    Animal Models: Female ICR (CD-1) mice, Hsd: Sprague-Dawley outbred rats, NSG mice(humanized mice), hu-PBL-SCID mice(humanized mice)
    Formulation: --
    Dosages: --
    Administration: i.v.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/27493273

    Pembrolizumab can apply to mice, rat, peripheral blood and other related assays (Only for Reference)

Product Details

CAS No. 1374853-91-4
Formulation PBS buffer, pH 7.2
Isotype Human IgG4
Source CHO cells
Storage Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

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Frequently Asked Questions

  • Question 1:

    How to store the antibody?

  • Answer:

    Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID