Pembrolizumab (anti-PD-1)

Catalog No.A2005 Synonyms: MK-3475, lambrolizumab Humanized and Non-humanized mouse model applicable

For research use only. Not for use in humans.

Pembrolizumab (anti-PD-1) is a potent, highly selective, fully humanized immunoglobulin (Ig) G4-kappa monoclonal antibody against PD-1 with potential immune checkpoint inhibitory and antineoplastic activities. MW : 146.286 KD.

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Cited by 3 publications

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Choose Selective PD-1/PD-L1 Inhibitors

Biological Activity

Description Pembrolizumab (anti-PD-1) is a potent, highly selective, fully humanized immunoglobulin (Ig) G4-kappa monoclonal antibody against PD-1 with potential immune checkpoint inhibitory and antineoplastic activities. MW : 146.286 KD.
Targets
PD-1 [1]
(Cell-free assay)
In vivo In addition to Melanoma and Lung carcinoma, pembrolizumab has also demonstrated efficacy in other advanced solid tumors and hematologic malignancies. Pembrolizumab had an acceptable side-effect profile and showed antitumor activity in patients with advanced non-small-cell lung cancer[2].

Protocol

Cell Research:
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  • Objective: To examine the effect of high-dose steroid therapy on lymphocyte proliferation/functionality after stimulation with anti-CD3 in the presence of pembrolizumab
    Cells: Human PBMCs
    Concentrations: 5 µg/mL
    Incubation Time: --
    Method: Isolated PBMCs were pre-treated with prednisone (1 nM - 1 mM) before anti-CD3 stimulation. Anti-CD3-stimulated cells were then incubated with pembrolizumab and their proliferative capacity and IL-2 secretion ability were measured by carboxyfluorescein succinimidyl ester (CFSE) staining and ELISA.
    Reference: http://ascopubs.org/doi/abs/10.1200/JCO.2017.35.15_suppl.e14587

    Pembrolizumab can apply to mice, rat, peripheral blood and other related assays (Only for Reference)

Animal Research:
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  • Objective: To evaluate the pharmacokinetics and biodistribution of 89Zr-labeled Pembrolizumab
    Animal Models: Female ICR (CD-1) mice, Hsd: Sprague-Dawley outbred rats, NSG mice(humanized mice), hu-PBL-SCID mice(humanized mice)
    Formulation: --
    Dosages: --
    Administration: i.v.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/27493273

    Pembrolizumab can apply to mice, rat, peripheral blood and other related assays (Only for Reference)

Product Details

Formulation PBS buffer, pH 7.2
Isotype Human IgG4
Source CHO cells
Storage Store the undiluted solution at 4 °C in the dark and avoid freezing

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Frequently Asked Questions

  • Question 1:

    How to store the antibody?

  • Answer:

    Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID