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Pembrolizumab (anti-PD-1)

Catalog No.A2005 Synonyms: MK-3475, lambrolizumab

For research use only.
Not for use in humans.

Pembrolizumab (anti-PD-1) is a potent, highly selective, fully humanized immunoglobulin (Ig) G4-kappa monoclonal antibody against PD-1 with potential immune checkpoint inhibitory and antineoplastic activities. MW : 146.286 KD.

Selleck's Pembrolizumab (anti-PD-1) has been cited by 26 publications

Purity & Quality Control

Choose Selective PD-1/PD-L1 Inhibitors

Biological Activity

Description

Targets

PD-1 ^[1]
(Cell-free assay)

In vivo

In addition to Melanoma and Lung carcinoma, pembrolizumab has also demonstrated efficacy in other advanced solid tumors and hematologic malignancies. Pembrolizumab had an acceptable side-effect profile and showed antitumor activity in patients with advanced non-small-cell lung cancer^[2].

Protocol (from reference)

Cell Research:	Objective: To examine the effect of high-dose steroid therapy on lymphocyte proliferation/functionality after stimulation with anti-CD3 in the presence of pembrolizumab Cells: Human PBMCs Concentrations: 5 µg/mL Incubation Time: -- Method: Isolated PBMCs were pre-treated with prednisone (1 nM - 1 mM) before anti-CD3 stimulation. Anti-CD3-stimulated cells were then incubated with pembrolizumab and their proliferative capacity and IL-2 secretion ability were measured by carboxyfluorescein succinimidyl ester (CFSE) staining and ELISA. Reference: http://ascopubs.org/doi/abs/10.1200/JCO.2017.35.15_suppl.e14587 Pembrolizumab can apply to mice, rat, peripheral blood and other related assays (Only for Reference)
Animal Research:	Objective: To evaluate the pharmacokinetics and biodistribution of 89Zr-labeled Pembrolizumab Animal Models: Female ICR (CD-1) mice, Hsd: Sprague-Dawley outbred rats, NSG mice(humanized mice), hu-PBL-SCID mice(humanized mice) Formulation: -- Dosages: -- Administration: i.v. Reference: https://www.ncbi.nlm.nih.gov/pubmed/27493273 Pembrolizumab can apply to mice, rat, peripheral blood and other related assays (Only for Reference)

Cell Research:

Objective: To examine the effect of high-dose steroid therapy on lymphocyte proliferation/functionality after stimulation with anti-CD3 in the presence of pembrolizumab
Cells: Human PBMCs
Concentrations: 5 µg/mL
Incubation Time: --
Method: Isolated PBMCs were pre-treated with prednisone (1 nM - 1 mM) before anti-CD3 stimulation. Anti-CD3-stimulated cells were then incubated with pembrolizumab and their proliferative capacity and IL-2 secretion ability were measured by carboxyfluorescein succinimidyl ester (CFSE) staining and ELISA.
Reference: http://ascopubs.org/doi/abs/10.1200/JCO.2017.35.15_suppl.e14587

Pembrolizumab can apply to mice, rat, peripheral blood and other related assays (Only for Reference)

Animal Research:

Objective: To evaluate the pharmacokinetics and biodistribution of 89Zr-labeled Pembrolizumab
Animal Models: Female ICR (CD-1) mice, Hsd: Sprague-Dawley outbred rats, NSG mice(humanized mice), hu-PBL-SCID mice(humanized mice)
Formulation: --
Dosages: --
Administration: i.v.
Reference: https://www.ncbi.nlm.nih.gov/pubmed/27493273

Pembrolizumab can apply to mice, rat, peripheral blood and other related assays (Only for Reference)

References	[1] Khoja L, et al. J Immunother Cancer. 2015, 3:36. [2] Garon EB, et al. N Engl J Med. 2015, 372(21):2018-28.

References

Product Details

CAS No.	1374853-91-4
Formulation	PBS buffer, pH 7.2
Isotype	Human IgG4
Source	CHO cells
Storage	Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

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Frequently Asked Questions

Question 1:
How to store the antibody?

Answer:
Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.