CAS No. 31430-18-9
Batch:
Purity:99.80
Chemical Structure
Check the
Nocodazole
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | methyl N-[6-(thiophene-2-carbonyl)-1H-benzimidazol-2-yl]carbamate | |
|---|---|---|
| CAS Number | 31430-18-9 | |
| Molecular Formula |
C14H11N3O3S |
|
| Molecular Weight (MW) | 301.32 | |
| SMILES | COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3 | |
| InChIKey | KYRVNWMVYQXFEU-UHFFFAOYSA-N |
Biological Activity
Nocodazole is a rapidly reversible inhibitor of microtubule polymerization that also targets Abl kinases, including Abl, Abl(E255K), and Abl(T315I). By disrupting microtubule dynamics, it arrests cells in the G2/M phase of the cell cycle and impairs mitotic spindle assembly. Downstream, nocodazole inhibits dynamic instability at low concentrations, activates apoptotic pathways, and induces cell death in malignant cells. [1][2]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
7.5 mg/mL
(24.89 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
| DMSO |
6 mg/mL
(19.91 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Pre-aliquoted for Longevity: Solid-state aliquots offer longer shelf life compared to stock solutions.
Looking for a specific aliquot size? Contact your local sales representative or orders@selleckchem.com.
Reference
Jump to
- Nocodazole Mechanism of Action: β-tubulin Inhibition in Cancer Cell Research
- Nocodazole Working Concentration | Cell Culture | Treatment
- Nocodazole Solubility in DMSO
- Nocodazole Stock Solution
- Nocodazole Storage
- Nocodazole Stability
- Nocodazole Combination Database
- Nocodazole Molecular Weight
- Nocodazole SMILES
- Nocodazole Chemical Structure (2D and 3D)
- Nocodazole IC50 for Cell-based and Cell-free Assays
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Note: Technical data last updated: Sep 1, 2026.