CAS No. 76931-93-6

Batch: Purity:99
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N-Succinimidyl-S-acetylthioacetate (SATA) Chemical Structure
Chemical Structure
Check the N-Succinimidyl-S-acetylthioacetate (SATA) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 76931-93-6
Molecular Formula

C8H9NO5S
Molecular Weight (MW) 231.23
SMILES CC(=O)SCC(=O)ON1C(=O)CCC1=O

Ordering Information

Biological Activity

N-Succinimidyl-S-acetylthioacetate (SATA) is an amine-reactive protein modification reagent that introduces protected sulfhydryl groups into primary amine-containing molecules. SATA reacts with lysine residues to form stable amide bonds, yielding acetylated thiol groups that are subsequently deprotected with hydroxylamine to generate free sulfhydryl groups. This modification enables site-specific bioconjugation and crosslinking with thiol-reactive targets, facilitating downstream applications in protein conjugation and assay development.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 46 mg/mL (198.93 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 4 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.