CAS No. 2756-87-8

Batch: Purity:99.59
Check CoA
Monomethyl Fumarate Chemical Structure
Chemical Structure
Check the Monomethyl Fumarate product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (E)-4-methoxy-4-oxobut-2-enoic acid
CAS Number 2756-87-8
Molecular Formula

C5H6O4
Molecular Weight (MW) 130.10
SMILES COC(=O)\C=C\C(O)=O
InChIKey NKHAVTQWNUWKEO-NSCUHMNNSA-N

Ordering Information

Biological Activity

Monomethyl Fumarate is a GPR109A agonist and the primary active metabolite of dimethyl fumarate that activates the Nrf2 antioxidant pathway. By promoting Nrf2 nuclear translocation, Monomethyl Fumarate upregulates cytoprotective antioxidant gene expression, effectively mitigating oxidative stress and mitochondrial dysfunction. Downstream, this activation suppresses apoptotic pathways and autophagy, conferring cytoprotection in cell-based models of neuroinflammation and neurodegeneration. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
🧮 Molarity Calculator

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 26 mg/mL (199.84 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 26 mg/mL
Water 20 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Jump to

Note: Technical data last updated: Sep 1, 2026.