CAS No. 329689-23-8

Batch: Purity:99.86
Check CoA
Monastrol Chemical Structure
Chemical Structure
Check the Monastrol product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name ethyl 4-(3-hydroxyphenyl)-6-methyl-2-sulfanylidene-3,4-dihydro-1H-pyrimidine-5-carboxylate
CAS Number 329689-23-8
Molecular Formula

C14H16N2O3S
Molecular Weight (MW) 292.35
SMILES CCOC(=O)C1=C(NC(=S)NC1C2=CC(=CC=C2)O)C
InChIKey LOBCDGHHHHGHFA-UHFFFAOYSA-N

Biological Activity

Monastrol is a cell-permeable small molecule inhibitor of kinesin-5 (KIF11) with an IC50 of. By specifically inhibiting the ATPase activity and motor function of KIF11, Monastrol disrupts mitotic spindle bipolarity without affecting tubulin polymerization. This inhibition blocks the separation of duplicated centrosomes, leading to cell cycle arrest in mitosis with characteristic monoastral spindles and halting cell proliferation. [1]

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 58 mg/mL (198.39 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 58 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
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Reference

[1] Mayer TU, et al. Science. 1999;286(5441):971-4.

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Note: Technical data last updated: Sep 1, 2026.