CAS No. 62-51-1

Batch: Purity:99.64
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Methacholine chloride Chemical Structure
Chemical Structure
Check the Methacholine chloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 62-51-1
Molecular Formula

C8H18NO2.Cl
Molecular Weight (MW) 195.69
SMILES CC(C[N+](C)(C)C)OC(=O)C.[Cl-]

Biological Activity

Methacholine chloride is a synthetic choline ester that functions as a non-selective muscarinic acetylcholine receptor agonist with minimal activity on nicotinic receptors. Activation of muscarinic receptors by methacholine stimulates parasympathetic signaling, promoting intracellular calcium mobilization and smooth muscle contraction, particularly in bronchial tissue. This compound is widely utilized to investigate airway hyperresponsiveness and cholinergic receptor-mediated signaling pathways. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 39 mg/mL (199.29 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 39 mg/mL
Ethanol 39 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Caulfield MP, et al. Pharmacol Rev. 1998;50(2):279-90.

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Note: Technical data last updated: Sep 1, 2026.